Antibacterial effect of acrylic bone cements loaded with drugs of different action’s mechanism

  • Lizette Morejón Alonso Center of Biomaterials, University of Havana, Havana, Cuba
  • Iran Fernández Torres National Center for Scientific Research, Havana, Cuba
  • Ángela M Zayas Tamayo National Center for Scientific Research, Havana, Cuba
  • Oscar Ernesto Ledea Lozano National Center for Scientific Research, Havana, Cuba
  • Ivette Durán Ramos Center of Biomaterials, University of Havana, Havana, Cuba
  • José Ángel Delgado García-Menocal Center of Biomaterials, University of Havana, Havana, Cuba
  • Nely Rios-Donato CUCEI, University of Guadalajara, Jalisco, Mexico
  • Eduardo Mendizábal CUCEI, University of Guadalajara, Jalisco, Mexico
Keywords: Antibiotic loaded bone cement, local antibiotic therapy, osteomyelitis, periprosthetic joint infections

Abstract

Introduction: Antibiotic-loaded bone cements of poly(methyl methacrylate) are considered as very useful biomaterials for the management of corporal deep osseous infections. However, the high prevalence of resistant germs and polymicrobial infections makes it necessary to search for new formulations of bone cements containing antibiotics for local antibacterial therapy. In this work, bone cements loaded with drugs with different mechanism of action were evaluated to determine its antibacterial effectiveness on Pseudomonas aeruginosa.

Methodology: Poly(methyl methacrylate) cements loaded with 10 wt.% of Oleozon®, mixtures of Ciprofloxacin/Meropenem and Ciprofloxacin/Meropenem/Oleozon® were prepared. The in vitro drugs release in water was followed by UV-Vis spectroscopy, and their antibacterial activity against Pseudomonas aeruginosa was evaluated for 11 days using the microdilution method.

Results: All the extracts demonstrated an inhibitory effect on the growth of the strain during the whole trial period. Extracts from cement with Oleozon® only presented a total antibacterial inhibitory effect during 20 hours for the extracts taken at day 1 while the extracts from the cements loaded with mixtures of Ciprofloxacin/Meropenem and Ciprofloxacin/Meropenem/Oleozon® showed complete inhibition of the growth of the microorganism, even at 11 days. At the end of the trial period, some of the drugs remained inside the matrices, indicating that they can be released for a longer time in treatments.

Conclusions: The results indicated a positive antibacterial effect by the combined used of the two or the three drugs tested against the Gram-negative bacilli Pseudomonas aeruginosa, so these proposal may be a valid alternative to be considered by surgeons.

Published
2019-06-30
How to Cite
1.
Morejón Alonso L, Fernández Torres I, Zayas Tamayo Ángela M, Ledea Lozano OE, Durán Ramos I, Delgado García-Menocal J Ángel, Rios-Donato N, Mendizábal E (2019) Antibacterial effect of acrylic bone cements loaded with drugs of different action’s mechanism. J Infect Dev Ctries 13:487-495. doi: 10.3855/jidc.10716
Section
Original Articles