Isolation of Cryptococcus species from the external environments of hospital and academic areas

  • Murilo de Oliveira Brito Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil
  • Meliza Arantes de Souza Bessa Institute of Biology, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil
  • Ralciane de Paula Menezes Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil
  • Denise Von Dolinger de Brito Röder Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil
  • Mário Paulo Amante Penatti Technical School of Health, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil
  • João Paulo Pimenta Checkup Medical Laboratory, Uberlandia, Minas Gerais, Brazil
  • Paula Augusta Dias Fogaça de Aguiar Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil
  • Reginaldo dos Santos Pedroso Faculty of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil
Keywords: Cryptococcus species, environmental health, opportunistic infection

Abstract

Introduction: Fungi of the genus Cryptococcus are cosmopolitan and may be agents of opportunistic mycoses in immunocompromised and sometimes immunocompetent individuals. Cryptococcus species are frequently isolated from trees and bird excreta in the environment and infection occurs by inhalation of propagules dispersed in the air. The aim was to investigate Cryptococcus species in bird excreta and tree hollows located in a university hospital area and in an academic area of a university campus.

Methodology: A total of 40 samples of bird excreta and 41 samples of tree hollows were collected. The identification of the isolates was done by classical methodology and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Results: Twenty (62.5%) isolates of Cryptococcus were found in bird excreta and 12 (37.5%) in tree hollows. C. laurentii (currently Papiliotrema laurentii) was the most frequent species in both samples, being found in 5 samples of excreta and in 8 tree hollows. The diversity of species found in excreta (C. laurentii, C. albidus [currently Naganishia albida], C. liquefaciens [currently N. liquefaciens], C. friedmanii [currently N. friedmannii] and others) was higher than in tree hollows (C. laurentii, C. flavescens [currently Papiliotrema flavescens], and other yeasts).

Conclusion: Many Cryptococcus species were isolated from excreta and tree hollows, and this fact is important for understanding the environmental epidemiology of those emerging pathogens for public health, as a way to implement surveillance actions and control of cryptococcosis.

Published
2019-06-30
How to Cite
1.
Brito M de O, Bessa MA de S, Menezes R de P, Röder DVD de B, Penatti MPA, Pimenta JP, Aguiar PADF de, Pedroso R dos S (2019) Isolation of Cryptococcus species from the external environments of hospital and academic areas. J Infect Dev Ctries 13:545-553. doi: 10.3855/jidc.10849
Section
Original Articles