Diagnosis of human sporotrichosis in Campos dos Goytacazes, Rio de Janeiro, Brazil

  • Adriana Jardim Almeida Center for Agricultural Science and Technology, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil
  • Edilbert Pelegrini Nahn Júnior Adult Health Department, Campos School of Medicine, Campos dos Goytacazes, RJ, Brazil
  • Olney Vieira da Motta Center for Agricultural Science and Technology, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil
  • Camila da Silva Lourenço Center for Agricultural Science and Technology, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil
  • Maria de Lourdes Amaral Bernardino Center for Agricultural Science and Technology, Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, RJ, Brazil
  • Gabriel Portal Barros Pellegrini Nahn Adult Health Department, Campos School of Medicine, Campos dos Goytacazes, RJ, Brazil
Keywords: Sporothrix, prevention, public health, zoonosis

Abstract

Introduction: Sporotrichosis is an infectious fungal zoonosis associated with traumatic implantation in the skin of dimorphic fungi of the Sporothrix schenckii complex. The objective of this study was to diagnose sporotrichosis in patients in the city of Campos dos Goytacazes, and to establish correlations between positive laboratory results and dermatological and topographical aspects of the lesions and personal traits of the patients, such as sex, age and profession.

Methodology: We collected samples from 22 patients with suspect lesions, which were sent to the laboratory for direct microscopic analysis after Gram staining, followed by mycological culture by seeding the material in 4% Sabouraud dextrose agar (Himedia®) supplemented with chloramphenicol (50 mg/Lt) and cycloheximide (400 mg/Lt - Sigma-Aldrich, USA). The dishes were incubated at 25-30oC. For confirmation of the diagnosis, the strains isolated in mycelial form were converted to yeast form by culture in yeast-peptone-dextrose (YPD) agar at 37oC for up to 15 days.

Results: The positive results demonstrated that the disease was most frequently diagnosed in women between the ages of 19 and 60 years, and that 18 (81.8%) of the patients reported having contact with pet cats. The occupations of those positive for sporotrichosis were not related to the disease. The upper limbs were the body region most often afflicted, with observation in the majority of cases of ulcerated lesions, although five patients also had nodular lesions. Additionally, the observation of lymphatic cords was frequent.

Conclusion: In recent years, sporotrichosis has been diagnosed with relative frequency in Campos dos Goytacazes, causing great concern among public health officials and practitioners.

Published
2019-08-31
How to Cite
1.
de Almeida AJ, Nahn Júnior EP, Vieira da Motta O, Lourenço C da S, Bernardino M de LA, Nahn GPBP (2019) Diagnosis of human sporotrichosis in Campos dos Goytacazes, Rio de Janeiro, Brazil. J Infect Dev Ctries 13:768-772. doi: 10.3855/jidc.11672
Section
Brief Original Articles