Relation of interleukin-1β gene to treatment response in chronic patients infected with HCV genotype 4

  • Moataza Hassan Omran Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
  • Noha E. Ibrahim Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
  • Samar S. Youssef Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
  • Basma E Fatouh Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
  • Wael Nabil Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
  • Magdy M El-Shami Faculty of Science, Menoufia University, Shibin El Kom, Egypt
  • Mostafa K El-Awady Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
Keywords: HCV, Interlukin-1β, interferon therapy, sustained virological response, single nucleotide polymorphism

Abstract

Introduction: Hepatitis C virus (HCV) infection results in chronic hepatitis in more than 70% of infected patients, while 20-30% of patients recover spontaneously. This strengthens the role of the host genetic factors in either spontaneous or drug-induced viral clearance. The aim of this study was to investigate the relationship between interleukin-1β +3953 gene polymorphism and the response to interferon therapy in chronic HCV patients infected with genotype 4.

Methodology: The interleukin-1β (+3953 C/T) (rs1143634) gene was amplified in 115 chronic HCV patients. Interleukin-1β single nucleotide polymorphism (SNP) plus several clinical and pathological factors were statistically analyzed in correlation with response to therapy.

Results: Genotypes C/T and T/T had a significant association with non-response to treatment compared to genotype C/C, which had a strong association with response to treatment (95% confidence; 6.4884-48.5818, p = 0.0001). Furthermore, analysis of allele frequency in this cohort revealed that the T allele is associated with non-response, higher fibrosis, and higher hepatic activity, while the C allele had a significant association with sustained virologic response lower fibrosis, and lower hepatic activity (p value = 0.0001).

Conclusion: This is the first study to examine the correlation between interleukin-1β (+3953 C/T) (rs1143634) gene polymorphism and the response of interferon therapy in genotype 4 HCV-infected patients. The results encourage further assessment of this SNP as a marker  to predict response to therapy and disease progression, which can have major implications in saving money, time, and in avoiding unnecessary adverse effects.

Author Biographies

Moataza Hassan Omran, Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
Assistant Professor of Biomedical BiotechnologyMicrobial Biotechnology Department,Genetic Engineering Division,National Research Center
Noha E. Ibrahim, Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
Researcher at Microbial Biotechnology Department,Genetic Engineering Division,National Research Center
Samar S. Youssef, Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
Assistant Professor of Biomedical BiotechnologyMicrobial Biotechnology Department,Genetic Engineering Division,National Research Center
Basma E Fatouh, Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
Researcher at Microbial Biotechnology Department,Genetic Engineering Division,National Research Center
Wael Nabil, Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
Researcher at Microbial Biotechnology Department,Genetic Engineering Division,National Research Center
Magdy M El-Shami, Faculty of Science, Menoufia University, Shibin El Kom, Egypt
Professorat at Menofia University
Mostafa K El-Awady, Genetic Engineering Division, National Research Centre, Dokki-Cairo, Egypt
Professor of Biomedical BiotechnologyMicrobial Biotechnology Department,Genetic Engineering Division,National Research Center
Published
2013-11-15
How to Cite
1.
Omran M, Ibrahim N, Youssef S, Fatouh B, Nabil W, El-Shami M, El-Awady M (2013) Relation of interleukin-1β gene to treatment response in chronic patients infected with HCV genotype 4. J Infect Dev Ctries 7:851-858. doi: https://doi.org/10.3855/jidc.3823
Section
Original Articles