Molecular epidemiology of coronavirus in faeces of Brazilian calves and Peruvian camelid herds

  • Camila B Rocha Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
  • Luz Alba A.M.G. Fornells Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
  • Miguel Rojas Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
  • Maíra Libetal Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro, Niterói, RJ, Brazil
  • Alberto Manchego Universidad Nacional Mayor de San Marcos, (FMV/UNMSM), Lima, Peru
  • Danilo Pezo Instituto Veterinario de Investigaciones Tropicales y de Altura (IVITA), Cuzco, Peru
  • Norma Santos Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil
Keywords: Coronavirus, Bovine, Alpaca, Diarrhoea, Epidemiology

Abstract

Introduction: The enteric disorders represent a serious hazard for bovine and camelid breeding. The aim of this study was to examine the frequency of detection and molecular characteristics of enteric coronavirus (CoV) infections in cattle, alpaca, and llama herds bred in family-based farms in Brazil and Peru.

Methodology: Stool samples were collected from calves from Brazil and camelids from Peru for detection and characterization of CoV by reverse transcription polymerase chain reaction (RT-PCR) and sequence analysis.

Results: 46.5% (47/101) samples from calves and 26.8% (70/261) from alpaca tested positive for CoV. All strains belong to lineage A1 of the Betacoronavirus genus. Phylogenetic analysis showed high identity between CoV strains detected in calves and alpacas.

Conclusions: This study characterised CoV strains from dairy cattle herds in the state of Rio de Janeiro, Brazil, and indicated that this virus is spread among the state herds. The results also indicate widespread circulation of CoV among the alpacas of Cuzco, Peru.

Published
2018-01-31
Section
Original Articles