Assessment of vaginal microbiota in Brazilian women with and without bacterial vaginosis and comparison with Nugent score

  • Laura Maria Andrade Oliveira Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Cláudio Galuppo Diniz Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Aline Augusta Sampaio Fernandes Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Daniele Maria Knupp Souza-Sotte Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Michelle Cristine Ribeiro Freitas Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Alessandra Barbosa Ferreira Machado Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
  • Vania Lucia Silva Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil
Keywords: Bacterial vaginosis, Gardnerella vaginalis, Lactobacillus, Vaginal microbiota, Nugent score

Abstract

Introduction: Bacterial vaginosis (BV) is characterized by the depletion of Lactobacillus spp. population and increase of other species, especially Gardnerella vaginalis and Atopobium vaginae. This study aimed to investigate the vaginal microbiota structure of Brazilian women with and without BV according to Nugent Score and to assess the correlation among Nugent score and the quantification of BV-associated bacteria.

Methodology: Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) assay was employed to characterize the vaginal microbiota structure. Quantification of Lactobacillus spp., G. vaginalis, A. vaginae, Mobiluncus sp. and M. hominis were determined by quantitative real-time PCR (qPCR).

Results: Clustering by PCR-DGGE revealed differences in microbial structure of the different patient groups. Gardnerella vaginalisA. vaginaeM. hominis and Mobiluncus sp. were detected at high loads in BV-associated microbiota. Quantification of Lactobacillus spp. showed an inverse correlation with Nugent score while the loads of G. vaginalisA. vaginaeM. hominis and Mobiluncus sp. indicated a direct correlation with this method.

Conclusions: Despite Nugent score is considered the gold standard for BV diagnosis, qPCR stands out as a useful tool for bacteria quantification and an alternative for BV diagnosis. Vaginal microbiota is a complex microbial community although there is a common core among BV and non-BV women. Investigation of vaginal microbiota structure may contribute to the development of tools for diagnosis improvement and therapeutic regimen optimization.

Author Biography

Vania Lucia Silva, Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil

Department of Parasitology, Microbiology and Immunology, Institute of Biological Sciences

Published
2018-02-28
How to Cite
1.
Oliveira L, Diniz C, Fernandes A, Souza-Sotte D, Freitas M, Machado A, Silva V (2018) Assessment of vaginal microbiota in Brazilian women with and without bacterial vaginosis and comparison with Nugent score. J Infect Dev Ctries 12:127-136. doi: https://doi.org/10.3855/jidc.9532
Section
Original Articles