Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

  • Hafeza Aftab Faculty of Medicine, Oita University, Yufu, Japan
  • Yoshio Yamaoka Faculty of Medicine, Oita University, Yufu, Japan
  • Faruque Ahmed Dhaka Medical College and Hospital, Dhaka, Bangladesh
  • Azad AK Khan BIRDEM, Dhaka, Bangladesh
  • Phawinee Subsomwong Faculty of Medicine, Oita University, Yufu, Japan
  • Muhammad Miftahussurur Faculty of Medicine, Oita University, Yufu, Japan
  • Tomohisa Uchida Faculty of Medicine, Oita University, Yufu, Japan
  • Hoda M Malaty Baylor College of Medicine, Houston, Texas, United States
Keywords: Bangladesh, Helicobacter pylori, prevalence, diagnosis test

Abstract

Introduction: Helicobacter pylori infection is associated with gastritis, peptic ulcer, and gastric cancer. We conducted a cross-sectional study to compare five diagnostic tests for H. pylori infection and studied the epidemiology of the infection in Bangladesh.

Methodology: Bangladeshi patients with dyspeptic symptoms referred for endoscopic examination were enrolled in this study. Each patient underwent upper endoscopic examination and four gastric biopsy specimens were taken. We used 5 tests for the diagnosis of H. pylori; culture, histology confirmed by immunohistochemistry, rapid urease test (RUT), urinary and serological test. Demographic and environmental variables were collected.

Results: A total of 133 patients participated in the study, 61 males and mean age 37.3 ± 12.3 years. We used the culture and/or histology results as the gold standard to estimate the sensitivity, specificity, positive and negative predictive values for the studied diagnostic tests. RUT, culture and histology had high sensitivity and specificity with moderate positive and negative likelihood ratio, whereas urine test and serology showed a good sensitivity and specificity but poor likelihood ratio. The overall prevalence of H. pylori among study subjects was 47% with no difference between gender and age groups.

Conclusions: The invasive tests showed better performance than noninvasive tests among Bangladeshi population. The overall prevalence of H. pylori was less than the previously reported in the region with no difference among all age groups.

Author Biographies

Hafeza Aftab, Faculty of Medicine, Oita University, Yufu, Japan

Department of Environmental and Preventive Medicine, Oita University, Faculty of Medicine, Yufu, Japan

Department of Gastroenterology, Dhaka Medical College and Hospital, Dhaka, Bangladesh

Faruque Ahmed, Dhaka Medical College and Hospital, Dhaka, Bangladesh

Department of Gastroenterology, Dhaka Medical College and Hospital, Dhaka, Bangladesh

Azad AK Khan, BIRDEM, Dhaka, Bangladesh

Department of Gastroenterology, BIRDEM, Dhaka, Bangladesh

Phawinee Subsomwong, Faculty of Medicine, Oita University, Yufu, Japan

Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Japan

Muhammad Miftahussurur, Faculty of Medicine, Oita University, Yufu, Japan

Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Japan

Gastroentero-Hepatology Division, Department of Internal Medicine, Faculty of Medicine-Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia

Tomohisa Uchida, Faculty of Medicine, Oita University, Yufu, Japan

Department of Molecular Pathology, Oita University Faculty of Medicine, Yufu, Japan

Hoda M Malaty, Baylor College of Medicine, Houston, Texas, United States

Department of Medicine, Gastroenterology and Hepatology Section, Baylor College of Medicine, Houston, Texas, USA

Published
2018-05-31
How to Cite
1.
Aftab H, Yamaoka Y, Ahmed F, Khan A, Subsomwong P, Miftahussurur M, Uchida T, Malaty H (2018) Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh. J Infect Dev Ctries 12:305-312. doi: https://doi.org/10.3855/jidc.9841
Section
Original Articles