@article{Said_Soliman_Shousha_Rashed_Elazm_Aamer_Kamel_Abdelsalam_2018, title={Interleukin-18 and its gene single nucleotide polymorphisms (SNPs) influence chronic hepatitis C progression}, volume={12}, url={https://jidc.org/index.php/journal/article/view/31851635}, DOI={10.3855/jidc.9813}, abstractNote={<p>Introduction: Interleukin-18 (IL-18) is a pro-inflammatory cytokine that is induced by hepatitis C virus (HCV) infection. Inter-individual variations of IL-18 gene expression may alter HCV-associated liver injury. Variable single nucleotide polymorphisms (SNPs) have been detected within IL-18 gene sequence. Quantitative assessment of IL-18 plasma level and detection of genotype frequencies of 2 functional polymorphisms of its gene (-607 C/A and -137 G/C) were done to assess their impact on the severity of chronic hepatitis C (CHC).</p> <p>Methodology: Cases group (I) comprised 110 treatment naïve CHC Egyptian patients (78 Males and 32 Females, mean age = 40.7 ± 11.8 years) who underwent routine laboratory investigations. Assessment of plasma level of IL-18 was done by enzyme-linked immunosorbent assay (ELISA), detection of IL-18 gene polymorphisms at positions -607 C/A and -137 G/C by polymerase chain reaction sequence specific polymorphism (PCR-SSP) analysis and Liver biopsy with METAVIR scoring were done. The control group (II) comprised 90 healthy participants.</p> <p>Results: Plasma levels of IL-18 were significantly higher in cases than the control group. We found a statistically highly significant (p < 0.001) positive correlation between IL-18 plasma level and both METAVIR necro-inflammatory grade and fibrosis stage. The A/A allele at -607 position was significantly more frequent (p < 0.05) in patients with F ≤ 1.</p> <p>Conclusions: Higher IL-18 plasma levels are found in CHC patients and positively correlate with the severity of liver disease. The presence of A/A allele at -607 position of IL-18 gene promoter is associated with milder liver disease.</p>}, number={04}, journal={The Journal of Infection in Developing Countries}, author={Said, Ebada M and Soliman, Mohamed S and Shousha, Hend Ibrahim and Rashed, Mohamed Sanad and Elazm, Abeer Abo and Aamer, Rania Zakaria and Kamel, Mai Hamed and Abdelsalam, Fatma Mohamed}, year={2018}, month={Apr.}, pages={257–264} }