TY - JOUR AU - Bonyadian, Mojtaba AU - Moshtaghi, Hamdallah AU - Nadi, Hanie PY - 2018/09/30 Y2 - 2024/03/29 TI - PCR detection of Vibrio cholerae, Escherichia coli, and Salmonella sp. from bottled drinking water in Iran JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 12 IS - 09 SE - Original Articles DO - 10.3855/jidc.10160 UR - https://jidc.org/index.php/journal/article/view/31999626 SP - 700-705 AB - <p>Introduction: The quality of drinking water has an important role in human health. This study was aimed to detect <em>Escherichia</em>. <em>coli</em>, <em>Salmonella sp.</em> and <em>Vibrio cholerae</em> from bottled drinking waters produced in Iran.</p><p>Methodology: A total of 240 samples of bottled water of different brands were collected for testing between March 2015 to December 2015 in Shahrekord-Iran. Samples were examined by polymerase chain reaction (PCR) combined with culture methods for the detection of <em>E</em>. <em>coli</em>, <em>Salmonella sp.</em>, and <em>V. cholerae</em>.</p><p>Results: The results of PCR revealed that the <em>uidA</em> gene from <em>E</em>. <em>coli</em>, <em>IpaB</em> gene from <em>Salmonella sp</em>, and <em>epsM</em> gene from <em>V. cholerae</em> were detected in 6 (2.5%), 1 (0.4 %), 0 (0%) of the samples, respectively. But in culture methods, only <em>E. coli </em>5 (2.1%) were isolated from the samples. The contamination with <em>E. coli</em> was significantly higher (P &lt; 0.05) in water produced during the hot seasons than the cold seasons.</p><p>Conclusions: This study confirmed the presence of <em>Escherichia coli</em> as the main microorganism in bottle drinking water in Iran. Also, our study showed that PCR can be used as a screening method for monitoring the enteric pathogens in drinking water.</p> ER -