TY - JOUR AU - Senok, Abiola Catherine AU - Somily, Ali AU - Raji, Muhabat AU - Garaween, Ghada AU - Kabil, Maha AU - Shibl, Atef AU - Monecke, Stefan AU - Ehricht, Ralf PY - 2018/05/31 Y2 - 2024/03/28 TI - Genotyping of Staphylococcus aureus associated with nasal colonization among healthcare workers using DNA microarray JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 12 IS - 05 SE - Original Articles DO - 10.3855/jidc.10328 UR - https://jidc.org/index.php/journal/article/view/31865293 SP - 321 - 325 AB - <p>Introduction: Healthcare workers (HCWs) colonized with <em>Staphylococcus aureus </em>may serve as a reservoir of infection. This study was carried to determine the genetic make-up of <em>S. aureus</em> nasal colonizers in HCWs.</p><p>Methodology: Nasal swabs were obtained from 93 HCWs and molecular characterization of identified <em>S. aureus</em> isolates was carried out using the StaphyType DNA microarray (Alere Technologies GmbH, Jena, Germany).</p><p>Results: Twenty-nine HCWs (31%) were colonized with <em>S. aureus</em> (MSSA = 23; MRSA = 6). Thus the overall MRSA carriage rate was 6.5% (n/N = 6/93) and 20.7% (n/N = 6/29) of those colonized with <em>S. aureus</em> harboured MRSA. The <em>S. aureus </em>isolates belonged to 16 clonal complexes (CC). MSSA isolates included three each for CC15, CC188, ST2867; two each for CC5, CC97, CC367 as well as one each for CC1, CC8, CC30, CC45, CC101, CC121, ST291/813 and CC1153. The staphylococcal cassette chromosome recombinase genes <em>ccrA-1</em>; <em>ccrB-1</em> and the fusidic acid resistance gene (<em>fus</em>C) were present in two MSSA isolates (CC1 and CC8). The six MRSA isolates included CC5-MRSA-[VI+<em>fusC</em>] (n = 2); one each of CC5-MRSA-V; CC22-MRSA-IV (<em>tst1<sup>+</sup></em>); CC80-MRSA-IV [<em>pvl</em><sup>+</sup>] (“European CA-MRSA Clone”) and CC97-MRSA-[V+<em>fusC</em>].</p><p>Conclusion: There is wide clonal diversity of <em>S. aureus</em> colonizers with associated high MRSA carriage among the HCWs. The presence of genetically stable MSSA isolates with the capability to transform into MRSA isolates is of concern.</p> ER -