TY - JOUR AU - Say Coskun, Umut Safiye AU - Caliskan, Emel AU - Copur Cicek, Asegul AU - Turumtay, Halbay AU - Sandalli, Cemal PY - 2019/01/31 Y2 - 2024/03/28 TI - β-lactamase genes in carbapenem resistance Acinetobacter baumannii isolates from a Turkish university hospital JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 13 IS - 01 SE - Original Articles DO - 10.3855/jidc.10556 UR - https://jidc.org/index.php/journal/article/view/32032023 SP - 50-55 AB - <p>Introduction: The spread of <em>Acinetobacter</em> <em>baumannii</em>, resistant to most of the available antimicrobial agents, is a serious health problem. The high rate of carbapenem resistance among <em>Acinetobacter</em> <em>baumannii</em> isolates is considered as a threat to public health. In this study, we aimed to determine the antibiotic resistance and related genes in carbapenem-resistant <em>Acinetobacter baumannii</em> isolates.</p><p>Methodology: Ninety six isolates of <em>A.</em> <em>baumannii</em> were included. Antimicrobial susceptibility was performed by Phoenix Automated System and disk diffusion method. Carbapenem resistane was characterized by scrneeing of resistance genes such as <em>bla</em><sub>TEM</sub>, <em>bla</em><sub>SHV</sub>, <em>bla</em><sub>CTX-M1-2</sub>, <em>bla</em><sub>PER</sub>, <em>bla</em><sub>VEB</sub>, <em>bla</em><sub>KPC</sub>, <em>bla</em><sub>GES</sub>, <em>bla</em><sub>NDM</sub>, <em>bla</em><sub>VIM</sub>, <em>bla</em><sub>IMP </sub>and <em>bla</em><sub>OXA23-24-51-58</sub> using multiplex polymerase chain reaction.</p><p>Results: Resistance for the levofloxacin, gentamicin, amikacin, and tigecycline were determined as 96.9%, 93.7%, 72.9% and 45.8% respectively. Colistin was the only susceptible antibiotic against all clinical isolates. All isolates were defined as multidrug resistance and of these, 31.2% were extensively drug-resistant (sensitive only to colistin). <em>Bla</em><sub>OXA-51­</sub> <em>&nbsp;</em>and<em> bla</em><sub>OXA-23 </sub>genes were detected in 100% strains while <em>bla</em><sub>TEM </sub>was found in only 2% strains. There was no amplification for the <em>bla</em><sub>SHV</sub>, <em>bla</em><sub>CTX-M1-2</sub>, <em>bla</em><sub>PER</sub>, <em>bla</em><sub>VEB</sub>, <em>bla</em><sub>KPC</sub>, <em>bla</em><sub>GES</sub> <em>bla</em><sub>NDM</sub>, <em>bla</em><sub>VIM</sub>, <em>bla</em><sub>IMP </sub>and <em>bla</em><sub>OXA24-58</sub> genes.</p><p>Conclusions: The high frequency of <em>bla</em><sub>OXA-23 </sub>and low frequency of <em>bla</em><sub>TEM</sub> gene was observed that indicate prevalence of a variety of <em>A.</em> <em>baumannii </em>strains. The rates of resistance genes vary from region to region. Studies are required for the prevention and control of <em>A. baumannii </em>infection and to formulate the strategies of antibiotic usage.</p> ER -