TY - JOUR AU - Zenati, Fatima AU - Barguigua, Abouddihaj AU - Nayme, Kaotar AU - Benbelaïd, Fethi AU - Khadir, Abdelmounaïm AU - Bellahsene, Chafika AU - Bendahou, Mourad AU - Hafida, Hassaïne AU - Timinouni, Mohammed PY - 2019/04/30 Y2 - 2024/03/29 TI - Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western Algeria JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 13 IS - 04 SE - Original Articles DO - 10.3855/jidc.10702 UR - https://jidc.org/index.php/journal/article/view/32045373 SP - 291-302 AB - <p>Introduction: The aim of this study is to assess the prevalence and molecular characterization of uropathogenic Extended spectrum β-lactamases (ESBLs) producing <em>Escherichia coli</em>.</p><p>Methodology: During 3 years, all hospitalized patients at the University-affiliated hospital of Tlemcen and presenting urinary tract infections caused by <em>E. coli </em>were considered as potential study participants. These <em>E. coli</em> isolates were examined phenotypically for ESBL production. ESBL strains were subjected to antimicrobial susceptibility testing and were investigated for the presence of plasmid mediated quinolone resistance genes, 16SrRNA methylase genes and virulence genes by using conventional PCR and DNA sequencing. The molecular characterization of ESBL strains was established by phylogenetic grouping method and ERIC-PCR.</p><p>Results: The overall prevalence of ESBL was 32.5%. The <em>bla</em><sub>CTX-M-15 </sub>was the most frequently detected in ESBL isolates, followed by <em>bla</em><sub>CTX-M-14</sub>, <em>bla</em><sub>CTX-M-28</sub>, <em>bla</em><sub>CTX-M-1</sub> and <em>bla</em><sub>SHV-12</sub> respectively. The plasmid-mediated quinolone resistance genes were detected in the 15 ESBL strains with the <em>aac(6’)-Ib-cr</em> gene was the most detected followed by <em>qnr</em>B1 and <em>qnr</em>A1 gene respectively. Among the 22 ESBL isolates resistant to gentamicin and amikacin, the 16SrRNA methylase genes were detected in 4 isolates. The <em>sfa</em> and <em>pap </em>virulent genes were founds in 26% and 22% of isolates receptively. The genotyping analysis of all strains revealed that almost were belonged to phylogenetic groups A<sub>1</sub> and A<sub>0</sub> and fourteen distinct clones.</p><p>Conclusion: The emergence of uropathogenic ESBL isolates and the high rate of <em>bla</em><sub>CTX-M </sub>are alarming in Algeria. Strict measure must be required to control the further spread of these strains in Algerian hospitals.</p> ER -