TY - JOUR AU - Garciglia Mercado, Carolina AU - Gaxiola Robles, Ramon AU - Ascencio, Felipe AU - Silva-Sanchez, Jesus AU - Estrada-Garcia, Maria Teresa AU - Gomez-Anduro, Gracia PY - 2020/05/31 Y2 - 2024/03/29 TI - Development of a LAMP method for detection of carbapenem-resistant Acinetobacter baumannii during a hospital outbreak JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 14 IS - 05 SE - Original Articles DO - 10.3855/jidc.11692 UR - https://jidc.org/index.php/journal/article/view/32525836 SP - 494-501 AB - <p>Introduction: Carbapenem-resistant <em>A. baumannii </em>(CRAB) represents a public health threat increasing worldwide. We assess the suitability of a loop-mediated isothermal amplification (LAMP) method for on-site screening of CRAB in a hospital facility.</p><p>Methodology: A set of six primers were designed for recognizing eight distinct sequences on six targets: <em>bla</em><sub>OXA-23-like</sub>, <em>bla</em><sub>OXA-24-like</sub>, <em>bla</em><sub>OXA-51-like</sub>, <em>bla</em><sub>OXA-58-like</sub>, <em>bla</em><sub>IMP</sub>, and <em>bla</em><sub>VIM</sub>. A LAMP method was developed, optimized and evaluated for the identification of CRAB in thirty-three environmental samples from an outbreak in an Intensive Care Unit (ICU) facility.</p><p>Results: The sensitivity of the LAMP assay for the detection of <em>A. baumannii</em> was ten-fold higher than the PCR assay (1.0 ng.µL-1). The LAMP assays showed a higher detection rate for CRAB samples and robust diagnosis performance in comparison to a conventional PCR, with clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 100% for <em>bla</em><sub>OXA-23-like</sub>, <em>bla</em><sub>OXA-51-like </sub>and <em>bla</em><sub>VIM</sub>.</p><p>Conclusions: The developed LAMP assays are powerful tools that can be useful in on-site screening of CRAB causing local outbreaks in clinics and hospitals facilities where costs and equipment restraints are imperative.</p> ER -