TY - JOUR AU - Jolaiya, Tolulope Funbi AU - Fowora, Muinah Adenike AU - Onyekwere, Charles AU - Ugiagbe, Rose AU - Agbo, Ifeanyi Ifeoma AU - Lesi, Olufunmilayo AU - Ndububa, Dennis Amojuayi AU - Adekanle, Olusegun AU - Njom, Henry Akum AU - Idowu, Ayodeji AU - Adeleye, Isaac Adeyemi AU - Bamidele, Moses AU - Ngoka, Favor Ndidiamaka AU - Palamides, Pia Fiona AU - Smith, Stella Ifeanyi PY - 2020/02/29 Y2 - 2024/03/28 TI - Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 14 IS - 02 SE - Original Articles DO - 10.3855/jidc.11746 UR - https://jidc.org/index.php/journal/article/view/32146450 SP - 162-168 AB - <p>Introduction: <em>Helicobacter pylori </em>is a principal cause of gastric cancer. The aim of this study was to determine the prevalence and contribution of duodenal ulcer promoting gene A (<em>dupA)</em>, the plasticity region genes and sigma factors in relation to their pathological expression of <em>H. pylori</em> infections in the Nigerian population.</p><p>Methodology: Polymerase Chain Reaction was used to analyze a total of forty-nine <em>H. pylori</em> strains isolated from patients attending various endoscopic units in tertiary hospitals in Nigeria for complete <em>dupA</em> (G27 variant), <em>jhp0917, jhp0918, </em>other plasticity region genes <em>jhp 914/917, jhp0914, jhp0940</em> and sigma factors.</p><p>Results: PCR results indicated that the prevalence of complete <em>dupA</em> (G27 variants), <em>jhp0917, jhp0918</em> and other plasticity region genes <em>jhp0914</em>, <em>jhp0914/0917</em> and <em>jhp0940</em> in the <em>H. pylori</em> strains were 4%, 53%, 88%, 73%, 12% and 0% respectively. The prevalence values of the sigma factors were 96%, 92%, 80% for <em>rpoN</em>, &nbsp;<em>fliA</em> and <em>rpoD</em> respectively. However, the endoscopic findings showed that erosion, normal mucosal, ulcer, hyperaemic stomach, mucosal atrophy and oedematous stomach in the patients where the <em>H. pylori</em> strains were isolated were 40.8%, 32.7%, 10.2%, 8.2%, 2.0% and 6.1% respectively. There was significant association between <em>jhp0917, jhp914/917</em> and <em>G27 </em>variant and the endoscopic findings, while other plasticity genes showed no association with the endoscopic findings.</p><p>Conclusion: These results suggest that the presence of <em>jhp0917, jhp0914/917</em> and G27 variant could be used as marker to predict the pathological effect of severity in Nigeria patients with <em>H. pylori</em> infection.</p> ER -