TY - JOUR AU - Eltai, Nahla AU - Al Thani, Asmaa A AU - Al-Hadidi, Sara H AU - Abdfarag, Elmoubasher A AU - Al-Romaihi, Hamad AU - Mahmoud, Mahmoud H AU - Alawad , Osama K AU - Yassine, Hadi M PY - 2020/02/29 Y2 - 2024/03/29 TI - Antibiotic resistance profile of commensal Escherichia coli isolated from healthy sheep in Qatar JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 14 IS - 02 SE - Original Articles DO - 10.3855/jidc.11827 UR - https://jidc.org/index.php/journal/article/view/32146447 SP - 138-145 AB - <p>Introduction: The uncontrolled antibiotics use in livestock is a leading factor for the emergence and spread of resistant bacteria from food animals to humans through the food chain. This study aims at evaluating the magnitude of the antimicrobial resistance (AMR) in food animals, acknowledging the lack of information on the prevalence of resistance in the veterinary field in Qatar.</p><p>Methodology: Rectal samples were collected from 171 sheep across three localities in Qatar between December 2016 and July 2017. These rectal samples were suspended in Phosphate buffer solution (PBS). Then streaked onto a selective CHROMagar <em>E. coli </em>medium plates and incubated at 37°C for 18 to 24 h. Isolated <em>E. coli</em> were tested for antibiotic susceptibility against 16 clinically- relevant antibiotics using the E-test method. Statistical analyses were performed using SPSS statistics 24.</p><p>Results: <em>E. coli</em> was isolated from 144 samples (84.2%), of which 90% were resistant to at least one antibiotic and 44% were multi-drug resistant (MDR). The highest resistance was against ciprofloxacin 69.4% (100), followed by nitrofurantoin 47.2% (68), trimethoprim/sulfamethoxazole 45.8% (66), cephalothin 43% (62) and amoxicillin/clavulanic acid 18% (26). Low resistance was reported to fosfomycin, amikacin and tigecycline 1.4% (2), 0.7% (1), and 0.7% (1), respectively.</p><p>Conclusions: We reported high MDR <em>E. coli</em> in rectal swabs of sheep in Qatar. Such resistant bacteria can potentially be transmitted to humans, resulting in public health concerns. This requires a quick response to develop and implement a stewardship program for the monitoring of antibiotic use in the veterinary in Qatar.</p> ER -