TY - JOUR AU - Suthum, Krairerk AU - Samosornsuk, Worada AU - Samosornsuk, Seksun PY - 2020/03/31 Y2 - 2024/03/29 TI - Characterization of katG, inhA, rpoB and pncA in Mycobacterium tuberculosis isolates from MDR-TB risk patients in Thailand JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 14 IS - 03 SE - Original Articles DO - 10.3855/jidc.11974 UR - https://jidc.org/index.php/journal/article/view/32235087 SP - 268-276 AB - <p>Introduction: Multidrug-resistant tuberculosis (MDR-TB) is commonly found in Thailand especially in the public health region 5, the Western region of Thailand. This study’s aim was to characterize <em>katG, inhA, rpoB</em> and <em>pncA</em> genes in <em>Mycobacterium tuberculosis.</em></p><p>Methodology: One hundred strains of <em>Mycobacterium tuberculosis</em> (MTB) were isolated from sputum samples of MDR-TB risk patients in the laboratory of the Office of Disease Prevention and Control 5<sup>th</sup> Ratchaburi province, Thailand from January to December 2015. Drug susceptibility testing (DST) was performed using a BACTEC MGIT 960 system. Furthermore, the genes <em>katG, inhA, rpoB</em> and <em>pncA</em> were characterized by DNA sequencing.</p><p>Results: Of a total of 100 MTB samples which underwent drug susceptibility testing, 42% showed isoniazid (INH) and rifampicin (RIF) resistance, and a further 25% showed INH mono-resistance (25%). The most common gene mutations found using DNA sequencing were <em>katG</em>_Ser315Thr (70%), <em>rpoB</em>_Ser531leu (81%) and <em>pncA</em>_Ile31Thr (84%). The common mutation of <em>pncA</em>_Ile31Thr substitution was detected in 26 of 91 (29%) pyrazinamide (PZA) susceptible isolates.</p><p>Conclusion: Using DNA sequencing to screen for gene mutations conferring drug resistance may be feasible and use less time than using DST to detect resistance patterns.</p> ER -