TY - JOUR AU - Hama-Soor, Taib Ahmed AU - Mohammed, Aram Ahmad AU - Weli, Sardar PY - 2021/10/31 Y2 - 2024/03/29 TI - Novel isolate of Cysticercus tenuicollis-Kalar isolate has been revealed in Kalar, Iraq JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 15 IS - 10 SE - Original Articles DO - 10.3855/jidc.12758 UR - https://jidc.org/index.php/journal/article/view/34780377 SP - 1532-1538 AB - <p>Introduction: Although <em>Cysticercus tenuicollis</em> is one of the most economic and veterinary important parasite in Iraq, scanty molecular characterization exists for this helminth. This study aimed to determine the prevalence and molecular description of <em>C. tenuicollis</em> isolates from sheep in Kalar district of Iraq.</p><p>Methodology: A total of 2,906 slaughtered sheep were examined post-mortem. Up to 20 samples of <em>C. tenuicollis</em> was extracted and amplified using mitochondrial COX1 gene.</p><p>Results: The overall prevalence rate was 6.88%, and female sheep recorded higher rate of infection (24.35%) than male (6.16%) with significant difference (<em>p</em>&lt;0.05). The molecular results showed 14 haplotypes for COX1 gene and the pairwise nucleotide variation among them was ranged from 0.2 to 2.6%. Twelve out of fourteen haplotypes of <em>C. tenuicollis</em> involving one to three base mutations were discovered in Kalar, Iraq for the first time and this could be a unique mutation internationally and did not registered previously. Eleven newly recorded haplotypes involved only one single mutation and the remaining one involved three mutations. Phylogenetic interpretation showed that <em>Cysticercus tenuicollis-</em>Kalar isolate were clustered in one clade, and closely related to isolates discovered in Nigeria, China, Turkey, Poland, and Iran.</p><p>Conclusions: This study provided a new record data on prevalence and discovered novel strains of <em>C. tenuicollis </em>in the study area for the first time named <em>Cysticercus tenuicollis-</em>Kalar isolate. Novel haplotypes might consider endemic genetic characterization of this metacestode. The present data may be useful to provide a good molecular background for future preventive and control programs.</p> ER -