TY - JOUR AU - Bello-López, Juan Manuel AU - Sanchez- Garibay, Carlos AU - Ibáñez-Cervantes, Gabriela AU - León-García, Gregorio AU - Gonzalez-Avila, Luis Uriel AU - Hernández-Cortez, Cecilia AU - Arzate Barbosa, Patricia AU - Castro-Escarpulli, Graciela PY - 2020/10/31 Y2 - 2024/03/30 TI - Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 14 IS - 10 SE - Original Articles DO - 10.3855/jidc.12966 UR - https://jidc.org/index.php/journal/article/view/33175710 SP - 1146-1154 AB - <p>Introduction: Intestinal and extraintestinal infections by <em>Aeromonas </em>spp., remain controversial, due to the existence of healthy carriers of <em>Aeromonas </em>spp. In children under five years old, the diarrhea of infectious origin constitutes the second cause of mortality and remains a major concern for public health. The aim of this work was to detect the pheno/genotype of β-lactamases and class 1 integrons in <em>Aeromonas</em> spp., strains isolated from pediatric patients in a tertiary referral hospital in Mexico.</p><p>Methodology: Sixty-six strains of <em>Aeromonas </em>spp., were isolated from clinical samples of pediatric origin and were identified by RFLP-PCR 16S rRNA. Resistance phenotype according to CLSI, genetic and phenotypic detection of extended-spectrum β-lactamases (ESBL) and metallo-b-lactamases (MBL) was performed. Finally, characterization of class 1 integrons was performed.</p><p>Results: <em>Aeromonas</em> spp., strains of diarrheic origin were more predominant. A wide heterogeneity was detected, where <em>A. caviae</em> was the predominant specie<em>. </em>Second-generation cephalosporins, fluoroquinolones, and nitrofurans had best antimicrobial activity; moreover, antibiotics of the β-lactamic and lincosamides families showed lower inhibitory activity. Phenotypically, prevalences of 4.55% and 3.03% were detected for MBL (intestinal origin) and ESBL (extraintestinal origin), respectively. <em>bla<sub>IMIS-cphA</sub></em> and <em>bla<sub>TEM-1</sub></em> genes, and nineteen class 1 integrons carrying two variants of cassettes corresponding to adenylyl transferases (<em>aadA</em>), and dihydrofolate reductases (<em>dfrA</em>). Monogenic array with <em>aadA1</em> cassette was predominantly.</p><p>Conclusions: ESBL and class 1 integrons, in <em>Aeromonas</em> collected from pediatric patients, determines a major detection challenge for the clinical microbiology laboratory and represents a remarkable epidemiological risk of nosocomial spread of multidrug-resistant determinants.</p> ER -