TY - JOUR AU - Hussain, Nazar AU - Tariq, Muhammad AU - Saris, Per Erik Joakim AU - Zaidi, Arsalan PY - 2021/01/31 Y2 - 2024/03/29 TI - Evaluation of the probiotic and postbiotic potential of lactic acid bacteria from artisanal dairy products against pathogens JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 15 IS - 01 SE - Original Articles DO - 10.3855/jidc.13404 UR - https://jidc.org/index.php/journal/article/view/33571152 SP - 102-112 AB - <p>Introduction: Probiotic and postbiotic potential of thirty-two strains of lactic acid bacteria (LAB), obtained earlier from artisanal dairy sources in Pakistan, have been investigated against major multi-drug resistant (MDR) and food borne pathogenic bacteria.</p><p>Methodology: LAB strains were identified by 16S rRNA gene sequencing and their antibacterial activity was assessed by the microdilution method. Four LAB isolates, <em>Weissella confusa</em> PL6, <em>Enterococcus faecium</em> PL7, and <em>Lactobacillus delbrueckii</em> PL11 and PL13 were shortlisted. Their ability to degrade lactose and safety for human consumption in terms of hemolysis and antibiotic susceptibility were assessed <em>in vitro</em>. The antibacterial components in the cell-free supernatants (CFSs) of isolate cultures were characterized biochemically by HPLC.</p><p>Results: Acid neutralization but not protease treatment abolished the antibacterial activity of CFSs. Lactic, acetic and propionic acids were the main acids in the CFSs, and acid production peaked in the stationary phase of growth. The antibacterial activity of the LAB cultures resulted from secretion of organic acids that lowered the pH. The strains exhibited variable ability to degrade lactose and were non-hemolytic and susceptible to the most common antibiotics.</p><p>Conclusions: These LAB strains are probiotic candidates for further investigation of their postbiotic role in naturally preserving processed foods and for attenuation of lactose intolerance.</p> ER -