TY - JOUR AU - Mohammad, Samira Metwally AU - Ali, Magda Saad AU - Abdel-Rahman, Sara Ahmed AU - Moustafa, Raghda Abdelrahman AU - Sarhan, Mohamed Hassan PY - 2021/10/31 Y2 - 2024/03/28 TI - Genotyping of Cryptosporidium species in children suffering from diarrhea in Sharkyia Governorate, Egypt JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 15 IS - 10 SE - Original Articles DO - 10.3855/jidc.14367 UR - https://jidc.org/index.php/journal/article/view/34780378 SP - 1539-1546 AB - <p>Introduction: The protozoan parasite <em>Cryptosporidium</em> is one of the principal reasons for childhood diarrhea around the world. This work aimed to differentiate <em>Cryptosporidium</em> species among children suffering from diarrhea in Sharkyia Governorate, Egypt.</p><p>Methodology: A total of 97 fecal specimens were taken from children suffering from diarrhea, attending Pediatric Clinics of Zagazig University and Al-Ahrar Hospitals. Full history was taken. Stool samples were examined microscopically using modified Ziehl–Neelsen stain for detection of <em>Cryptosporidium</em> oocysts. To identify <em>Cryptosporidium</em> genotypes, positive samples were then subjected to nested Polymerase chain reaction-restriction fragment length polymorphism targeting <em>Cryptosporidium</em> oocyst wall protein gene.</p><p>Results: The overall detection rate was 27.8% (27/97) using modified Ziehl–Neelsen stain staining method. Using nested polymerase chain reaction, the gene was amplified in 85.2% (23/27). Restriction fragment length polymorphism analysis revealed that 65.2% (15/23) were <em>Cryptosporidium hominis</em>, 30.4% (7/23) were <em>Cryptosporidium parvum,</em> and one sample was not typed (4.4%). The significant risk factors associated with <em>Cryptosporidium</em> infection in children were animal contact and residence in rural areas.</p><p>Conclusions: <em>Cryptosporidium</em> is a common enteric parasite affecting children in Sharkyia Governorate, Egypt, with the predominance of <em>C. hominis</em> genotype in children.</p> ER -