TY - JOUR AU - Yandag, Munkhdelger AU - Tsend-Ayush, Altansukh AU - Gunregjav, Nyamaa AU - Erdenebayar, Otgontsetseg AU - Byambadorj, Bayarlakh AU - Juniichiro, Nishi AU - Jav, Sarantuya PY - 2023/02/28 Y2 - 2024/03/28 TI - Detection and antibiotic resistance of diarrheagenic Escherichia coli from patients with diarrhea in Ulaanbaatar, Mongolia JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 17 IS - 02 SE - Original Articles DO - 10.3855/jidc.17256 UR - https://jidc.org/index.php/journal/article/view/36897900 SP - 202-209 AB - <p>Introduction: Diarrheal diseases are common with worldwide distribution, and diarrheagenic <em>Escherichia coli</em> (DEC) strains are the main causative agents. The present study aimed to define the association of various pathotypes of <em>E. coli</em> from diarrheal patients in Mongolia.</p><p>Methodology: A total of 341 <em>E. coli</em> strains were isolated from the stool of diarrheal patients. Bacterial susceptibility to antimicrobial agents was determined by the Kirby Bauer disk diffusion method. DEC isolates were identified by HEp-2 cell adherence assay and multiplex polymerase chain reaction (PCR).</p><p>Results: DEC pathogens were detected in 53.7% of 341 <em>E. coli</em> isolates. Enteroaggregative <em>E. coli</em> (EAEC) was the most common DEC pathotype identified by HEp-2 adherence assay and multiplex PCR methods in 97 samples (28.4%), followed by atypical enteropathogenic <em>E. coli</em> (EPEC) in 50 samples (14.7%), diffusely adherent <em>E. coli</em> (DAEC) in 25 samples (7.3%), enterohaemorrhagic <em>E. coli</em> (EHEC) in 6 samples (1.8%), enterotoxigenic <em>E. coli</em> (ETEC) in 4 samples (1.2%), and enteroinvasive <em>E. coli</em> (EIEC) in 1 sample (0.3%). DEC strains had &gt; 50% antibiotic resistance against cephalothin, ampicillin, and trimethoprim/sulfamethoxazole. All tested DEC strains were susceptible to imipenem. Among the 183 DEC strains, 27 (14.8%) were extended spectrum beta-lactamase producing isolates, and 125 (68.3%) isolates were multiple drug resistant.</p><p>Conclusions: We have identified six pathotypes of DEC from the clinical isolates tested and concluded that a high prevalence of antimicrobial resistance was observed in these pathotypes. EAEC was the most common pathotype identified and this is the first report of EHEC identification in Mongolia.</p> ER -