TY - JOUR AU - Ogbolu, David Olusoga AU - Daini, Oluwole Adebayo AU - Ogunledun, Afolabi AU - Terry Alli, Oyebode Armstrong AU - Webber, Mark Alexander PY - 2013/05/13 Y2 - 2024/03/29 TI - Dissemination of IncF plasmids carrying beta-lactamase genes in Gram-negative bacteria from Nigerian hospitals JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 7 IS - 05 SE - Original Articles DO - 10.3855/jidc.2613 UR - https://jidc.org/index.php/journal/article/view/23669427 SP - 382-390 AB - <p>Introduction: Production of beta-lactamases is the predominant cause of resistance to beta-lactam antibiotics in Gram-negative bacteria. We investigated the diversity of plasmid-borne beta-lactamase genes and replicon type of the plasmids carrying the respective genes in Gram-negative bacteria recovered from clinical infection in Nigerian hospitals.</p> <p>Methodology: A total of 134 Gram-negative bacteria of 13 species were analyzed for antimicrobial susceptibility, phenotypic and genotypic detection of various beta-lactamases, and plasmid analysis, including replicon typing.</p> <p>Results: Of the 134 isolates, 111 (82.8%) contained beta-lactamases, while 28 (20.9%) carried extended-spectrum beta-lactamases. PCR and sequencing identified TEM-1<em> </em>in 109 isolates (81.3%), SHV-1<em> </em>in 33 isolates (24.6%), OXA-1<em> </em>in 15 isolates (11.2%) and CTX-M<em> </em>enzymes (24 CTX-M-15<em> </em>and 1 CTX-M-3) in 25 isolates (18.7%). Multiplex PCR showed that 6 isolates carried plasmidic AmpCs (ACT-1, DHA-1 and CMY-2); these enzymes were detected only in isolates possessing CTX-M beta-lactamases. Of 13 (76.9%) representative plasmids investigated in detail, 9 (69.2%) were self-transferable when selected by a beta-lactam and the plasmids once transferred coded for beta-lactam resistance. Replicon typing indicated IncF as the common vector encoding for beta-lactamases.</p> <p>Conclusions: The study showed a diversity of beta-lactamase genes disseminated by conjugative IncF plasmids in Gram-negative bacteria; TEM-1, SHV-1, OXA-1, CTX-M-15, CTX-M-3and plasmidic AmpC enzymes are in common circulation in Nigeria.</p> ER -