TY - JOUR AU - Assis, Flávia E.A. AU - Wolf, Suélen AU - Surek, Monica AU - De Toni, Fabiana AU - Souza, Emanuel M AU - Pedrosa, Fábio O AU - Farah, Sônia M. S. S. AU - Picheth, Geraldo AU - Fadel-Picheth, Cyntia M.T. PY - 2014/12/15 Y2 - 2024/03/28 TI - Impact of Aeromonas and diarrheagenic Escherichia coli screening in patients with diarrhea in Paraná, southern Brazil JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 8 IS - 12 SE - Brief Original Articles DO - 10.3855/jidc.4434 UR - https://jidc.org/index.php/journal/article/view/25500659 SP - 1609-1614 AB - <p class="SmallText">Introduction: A wide diversity of bacterial agents may cause diarrhea, presenting challenges to clinical laboratories to define a diagnosis. Considering that most stool cultures are negative, we screened stool samples from patients with diarrhea for the presence of 14 bacterial enteropathogens, aiming to establish which of them should be included in routine stool analysis.</p> <p class="SmallText">Methodology: Stool samples from 400 patients with diarrhea were analyzed for the presence of <em>Salmonella</em>, <em>Shigella</em>, <em>Campylobacter</em>, <em>Aeromonas</em>, <em>Plesiomonas shigelloides</em>, <em>Vibrio</em>, <em>Yersinia</em> <em>enterocolitica</em>, and diarrheagenic <em>Escherichia</em> <em>coli</em> using conventional microbiological methods and PCR. Two distinct samples were studied; one included predominantly patients involved in outbreaks, and the other patients of low socioeconomic status presenting sporadic cases of diarrhea.</p> <p class="SmallText">Results: In total, 86 cultures (21.5%) were positive. Mixed infections were found in five patients, leading to recovery of 91 strains of enteropathogenic bacteria: <em>Salmonella</em> Enteritidis (9.2%), <em>Aeromonas</em> (7.2%), diarrheagenic <em>E. coli</em> (5.2%), and <em>C. jejuni </em>(1%). However, <em>Salmonella</em> predominated, with 11.5% frequency in diarrhea outbreaks, while<em> Aeromonas </em>predominated among patients of low socioeconomic status, with 14.6% frequency.</p> <p class="SmallText">Conclusion: <em>Aeromonas</em> and diarrheagenic <em>E. coli</em>, which are not routinely screened for, deserve to be included in laboratory screening panels.</p> ER -