TY - JOUR AU - Begaydarova, Rozalia AU - Yukhnevich, Yekaterina AU - Babenko, Dmitry AU - Kaliyeva, Sholpan AU - Azizov, Iliya AU - Muldaeva, Gulmira AU - Omarkulov, Baurzhan PY - 2015/05/18 Y2 - 2024/03/29 TI - Determination of PFOR gene expression in strains of G. intestinalis with different inhibitory concentrations of metronidazole JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 9 IS - 05 SE - Original Articles DO - 10.3855/jidc.5768 UR - https://jidc.org/index.php/journal/article/view/25989172 SP - 519-523 AB - <p class="SmallText">Introduction: <em>Giardia intestinalis</em> is the most important and common diarrhea-causing parasitic protozoa worldwide with growing clinical relevance in public health. There are many documented cases of <em>G. intestinalis</em> resistance to metronidazole (MZ). Pyruvate: ferredoxin oxidoreductase (PFOR), the membrane-localized enzyme, plays a key role in the development of resistance to drugs. The aim of the present study was to evaluate the difference in the levels of PFOR gene expression between MZ-resistant and MZ-susceptible strains of <em>G. intestinatlis</em>.</p> <p class="SmallText">Methodology: From 159 samples with <em>G. intestinalis</em> cysts, 48 strains were successfully cultivated. Using specific pair primers, PFOR gene expressions were estimated in different groups of <em>Giardia</em>. The polymerase chain reaction (PCR) data were analyzed with Bayesian analysis of qRT-PCR data using MCMC.qpcr package, with relative expression software tool (REST) and quantitative PCR CopyCount web source.</p> <p class="SmallText">Results: In the group of <em>Giardia</em> with minimum inhibitory concentration (MIC) of 6.3 µM, the level of PFOR gene expression was downregulated and compared with controls, differed by 1.5 to 2.8 times. At the same time, there was no significant difference in PFOR gene expression between the control (susceptible) group and the group with MIC of 3.2 µM.</p> <p class="SmallText">Conclusions: Though there is association between PFOR gene expression and metronidazole resistance of <em>Giardia intestinalis</em>, the level of PFOR gene expression cannot be a strong genetic marker to predict level of resistance to metronidazole based on MICs.</p> ER -