TY - JOUR AU - Ramis, Ivy Bastos AU - Vianna, Júlia Silveira AU - Halicki, Priscila Cristina Bartolomeu AU - Lara, Caroline AU - Tadiotto, Thássia Fernanda AU - da Silva Maciel, João Batista AU - Gonçalves, Carla Vitola AU - von Groll, Andrea AU - Dellagostin, Odir Antônio AU - da Silva, Pedro Eduardo Almeida PY - 2015/09/29 Y2 - 2024/03/29 TI - Relationship of interleukin-1B gene promoter region polymorphism with Helicobacter pylori infection and gastritis JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 9 IS - 10 SE - Original Articles DO - 10.3855/jidc.6123 UR - https://jidc.org/index.php/journal/article/view/26517486 SP - 1108-1116 AB - <p class="SmallText">Introduction:<em> Helicobacter pylori</em> infection is associated with gastritis, peptic ulcer disease and gastric carcinoma. The severity of damage is determined by the interplay between environmental/behavioral factors, bacterial pathogenicity genes and host genetic polymorphisms that can influence the secretion levels of inflammatory cytokines. Accordingly, this study aimed to identify polymorphisms in the <em>IL-1B </em>and <em>IL-1RN </em>genes and their associations with <em>H. pylori </em>infection, <em>cagA</em> gene of <em>H. pylori,</em> and gastroduodenal diseases.</p> <p class="SmallText">Methodology: Gastric biopsy samples from 151 patients infected with <em>H. pylori</em> and 76 uninfected individuals were analyzed. <em>H. pylori </em>infection was diagnosed by histology and PCR. Polymorphisms at positions -511, -31 and +3954 of the <em>IL-1B </em>gene were detected by PCR-RFLP, and an analysis of the VNTR polymorphism of the <em>IL-1RN</em> gene was performed by PCR.</p> <p class="SmallText">Results: It was observed that the presence of the T/T genotype at position -511 and the C/C genotype at position -31 were associated with <em>H. pylori</em> infection and with an increased risk of gastritis in <em>H. pylori-</em>positive patients. Additionally, strains from patients <em>H. pylori</em>-positive carrying the <em>cagA</em> gene was significantly related with the T/T genotype at position -511 of <em>IL-1B</em>.  No association of polymorphisms at position +3954 of <em>IL-1B</em> and in the <em>IL-1RN</em> with <em>H. pylori</em> infection and with risk of severe gastric diseases was found.</p> <p class="SmallText">Conclusions: We demonstrated that polymorphisms in the promoter region of the <em>IL-1B</em> gene (at positions -511 and -31) are associated with an enhanced risk of <em>H. pylori</em> infection as well as gastritis in <em>H. pylori-</em>positive patients.</p> ER -