TY - JOUR AU - Shome, Rajeswari AU - Krithiga, Natesan AU - Shankaranarayana, Padmashree B AU - Jegadesan, Sankarasubramanian AU - Udayakumar S, Vishnu AU - Shome, Bibek Ranjan AU - Saikia, Girin Kumar AU - Sharma, Narendra Kumar AU - Chauhan, Harshad AU - Chandel, Bharat Singh AU - Jeyaprakash, Rajendhran AU - Rahman, Habibur PY - 2016/03/31 Y2 - 2024/03/28 TI - Genotyping of Indian antigenic, vaccine, and field Brucella spp. using multilocus sequence typing JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 10 IS - 03 SE - Original Articles DO - 10.3855/jidc.6617 UR - https://jidc.org/index.php/journal/article/view/27031455 SP - 237-244 AB - <p class="SmallText">Introduction: Brucellosis  is one of the most important zoonotic diseases that affects  multiple livestock species and causes great economic losses. The highly conserved genomes of <em>Brucella</em>,<em> </em>with &gt; 90% homology among species, makes it important to study the genetic diversity circulating in the country.</p> <p class="SmallText">Methodology: A total of 26 <em>Brucella</em> spp. (4 reference strains and 22 field isolates) and 1 <em>B. melitensis </em>draft genome sequence from India (<em>B. melitensis </em>Bm IND1) were included for sequence typing. The field isolates were identified by biochemical tests and confirmed by both conventional and quantitative polymerase chain reaction (qPCR)<em> </em>targeting <em>bcsp</em> 31Brucella genus-specific marker. <em>Brucella</em> speciation and biotyping was done by Bruce ladder, probe qPCR, and AMOS PCRs, respectively, and genotyping was done by multilocus sequence typing (MLST).</p> <p class="SmallText">Results: The MLST typing of 27 <em>Brucella</em> spp. revealed five distinct sequence types (STs); the <em>B. abortus </em>S99 reference strain and 21 <em>B. abortus </em>field isolates belonged to ST1. On the other hand, the vaccine strain <em>B. abortus </em>S19 was genotyped as ST5. Similarly, <em>B. melitensis</em><em> </em>16M reference strain and one <em>B. melitensis </em>field isolate were grouped into ST7. Another <em>B. melitensis </em>field isolate belonged to ST8 (draft genome sequence from India), and only <em>B. suis</em> 1330 reference strain was found to be ST14.</p> <p class="SmallText">Conclusion: The sequences revealed genetic similarity of the Indian strains to the global reference and field strains. The study highlights the usefulness of MLST for typing of field isolates and validation of reference strains used for diagnosis and vaccination against brucellosis.</p> ER -