TY - JOUR AU - Ammar, Ahmed Mohamed AU - Mohamed, Adel Attia AU - Abd El-Hamid, Marwa Ibrahim AU - El-Azzouny, Mona Mohamed PY - 2016/04/28 Y2 - 2024/03/28 TI - Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 10 IS - 04 SE - Original Articles DO - 10.3855/jidc.7437 UR - https://jidc.org/index.php/journal/article/view/27130994 SP - 337-346 AB - <p class="SmallText">Introduction: <em>Salmonella</em> serovars are one of the primary foodborne pathogens. Poultry consumption is responsible for the majority of disease cases worldwide. The prevalence of virulence determinants among <em>Salmonella</em> serovars appears to be lacking in Egypt. Therefore, this study investigated the occurrence, antibiotic resistance patterns, and virulence gene profiling of <em>Salmonella</em> serovars in broilers.</p> <p class="SmallText">Methodology: Three hundred samples from broiler chickens were examined for the presence of <em>Salmonella</em> by standard microbiological techniques. All <em>Salmonella</em> isolates were tested for their sensitivity against ten antibiotics and subjected to virulence genotyping by polymerase chain reaction (PCR).</p> <p class="SmallText">Results: The overall isolation percentage of <em>Salmonella</em> was 17%. Seven different serovars were found, with the main one being <em>Salmonella</em> Typhimurium (52.94%). <em>Salmonella</em> isolates were sensitive to most of the tested antibiotics, but they exhibited absolute resistance against amoxicillin/clavulanic acid. Nine <em>Salmonella</em> strains (52.94%) were resistant to at least three antibiotics. Further PCR investigations into 17 <em>Salmonella</em> strains revealed different distribution patterns of eight virulence determinants among the isolates. The <em>inv</em>A gene was the most prevalent one (100%), followed by <em>hil</em>A (88.24%), <em>stn</em> (58.82%), and <em>fli</em>C genes (52.94%), while each of <em>sop</em>B and <em>pef</em>A genes had a similar prevalence (41.18%), and <em>sef</em>C and <em>spv</em>C genes had the lowest prevalence (11.76 and 5.88%, respectively). PCR genotyping allowed grouping of <em>Salmonella</em> strains into ten genetic profiles.</p> <p class="SmallText">Conclusions: These results will help in understanding the spread of virulence genotypes and antibiotic resistance among <em>Salmonella </em>serovars in broilers.</p> ER -