TY - JOUR AU - Gangoue-Pieboji, Joseph AU - Eze, Noelly AU - Ngongang Djintchui, Arnaud AU - Ngameni, Bathélémy AU - Tsabang, Nolé AU - Pegnyemb, Dieudonné Emmanuel AU - Biyiti, Lucie AU - Ngassam, Pierre AU - Koulla-Shiro, Sinata AU - Galleni, Moreno PY - 2009/10/22 Y2 - 2024/03/28 TI - The in-vitro antimicrobial activity of some medicinal plants against β-lactam-resistant bacteria JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 3 IS - 09 SE - Original Articles DO - 10.3855/jidc.77 UR - https://jidc.org/index.php/journal/article/view/19858568 SP - 671-680 AB - <p>Background: In effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria.</p> <p>Methodology: The antimicrobial activities of plant extracts were evaluated against clinically proved beta-lactam-resistant bacteria (<em>Klebsiella pneumoniae</em>, <em>Klebsiella </em><em>oxytoca</em>, <em>Enterobacter cloacae</em>, <em>Serratia marcescens</em>, <em>Acinetobacter baumannii</em>, <em>Staphylococcus aureus</em> and <em>Enterococcus</em> sp.) and reference strains of bacteria (<em>Escherichia coli</em> ATCC 35218, <em>Enterobacter aerogenes</em> ATCC 29751, <em>E. aerogenes</em> ATCC 13048, <em>Pseudomonas aeruginosa</em> ATCC 27853 and <em>Enterococcus hirae</em> ATCC 9790) by using disc-diffusion and agar-dilution assays.</p> <p>Results: The crude plant extracts demonstrated broad spectrum activity against all bacteria tested with inhibition zones in the range of 8-30 mm. The minimal inhibitory concentration (MIC) values of different plant extracts against the tested bacteria were found to range from ≤ 0.3 to ≥ 10 mg ml<sup>-1</sup>. The most active plant extracts were from <em>Dortenia picta</em> and <em>Bridelia micrantha</em> (MIC: 1.25-10 mg ml<sup>-1</sup>) on beta-lactam-resistant Gram-negative bacilli and the extracts from <em>B. micrantha</em>, <em>Mallotus </em><em>oppositifolius</em>, <em>Garcinia lucida</em>, <em>Garcinia. kola</em>, <em>Campylospermum densiflorum</em> (leaves) and <em>C. zenkeri</em> (root) on beta-lactam-resistant Gram-positive cocci (MIC: ≤ 0.3-5 mg ml<sup>-1</sup>).</p> Conclusion: Of the 17 plant extracts studied, seven showed good antimicrobial activity against the tested bacteria. The stem bark of <em>B. micrantha</em> and the leaves of <em>D. picta</em> were most active towards beta-lactamase producing Gram-negative bacilli. This study shows that medicinal plants could be sources of compounds which can be used to fight against beta-lactam resistant bacteria. <!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-fareast-font-family:Calibri; mso-bidi-font-family:"Times New Roman"; mso-fareast-language:EN-US;} span.bold1 {mso-style-name:bold1; mso-style-unhide:no; font-weight:bold;} span.h5-inline {mso-style-name:h5-inline; mso-style-unhide:no;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ascii-font-family:Calibri; mso-fareast-font-family:Calibri; mso-hansi-font-family:Calibri;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 70.85pt 70.85pt 70.85pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --><!--[if gte mso 10]> <mce:style><! /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Tableau Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} > <! [endif] > <p class="MsoNormalCxSpFirst" style="text-align: justify; line-height: 200%;" mce_style="text-align: justify; line-height: 200%;"><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-GB">Background: In efforts to fight against bacteria resistance to antibiotics, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against β-lactam-resistant bacteria.</span><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-GB"> </span></p> <p class="MsoNormalCxSpMiddle" style="text-align: justify; line-height: 200%;" mce_style="text-align: justify; line-height: 200%;"><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-GB">Methodology: The antimicrobial activities of plants extracts were evaluated against β-lactam-resistant clinical (<i>Klebsiella pneumoniae</i>, <i>Kl. </i></span><i><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-US">Oxytoca</span></i><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-US">, <i>Enterobacter cloacae</i>, <i>Serratia marcescens</i>, <i>Acinetobacter baumannii</i>, <i>Staphylococcus aureus</i> and <i>Enterococcus</i> sp.) </span><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-GB">and reference (<i>Escherichia coli</i> ATCC 35218, <i>Enterobacter aerogenes</i> ATCC 29751, <i>E. aerogenes</i> ATCC 13048, <i>Pseudomonas aeruginosa</i> ATCC 27853 and <i>Enterococcus hirae</i> ATCC 9790) strains by using disc-diffusion and agar-dilution assays. </span></p> <p class="MsoNormalCxSpMiddle" style="text-align: justify; line-height: 200%;" mce_style="text-align: justify; line-height: 200%;"><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-GB">Results: The crude plant extracts <span style="color: #231f20;" mce_style="color: #231f20;">demonstrated broad spectrum activity against all bacteria tested with inhibition zones in the range of 8-30 mm. The minimal inhibitory concentration (MIC) values of different plant extracts against the tested bacteria were found to range from ≤ 0.3 to ≥ 10 mg</span> ml<sup>-1</sup><span style="color: #231f20;" mce_style="color: #231f20;">. The most active plant extracts were from <i>Dortenia picta</i> and <i>Bridelia micrantha</i> (MIC: 1.25-10 mg </span>ml<sup>-1</sup><span style="color: #231f20;" mce_style="color: #231f20;">) on β-lactam-resistant Gram-negative bacilli and </span>the extracts from <i>B. micrantha</i>, <i>Mallotus </i></span><span class="bold1"><i><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-GB">oppositifolius</span></i></span><span style="font-size: 12pt; line-height: 200%; font-family: " mce_style="font-size: 12pt; line-height: 200%; font-family: " lang="EN-GB">, <i>Garcinia lucida</i>, <i>G. kola</i>, <i>Campylospermum densiflorum</i> (leaves) and <i>C. zenkeri</i> (root) on β-lactam-resistant Gram-positive cocci (MIC: ≤ 0.3-5 mg ml<sup>-1</sup>). </span></p> <span style="font-size: 12pt; line-height: 115%; font-family: " mce_style="font-size: 12pt; line-height: 115%; font-family: " lang="EN-GB">Conclusion: Of the 17 plant extracts studied, seven showed good antimicrobial activity against the tested bacteria. The stem bark of <i>B. micrantha</i> and the leaves of <i>D. picta</i> were most active towards β-lactamase producing Gram-negative bacilli. </span><span class="h5-inline"><span style="font-size: 12pt; line-height: 115%; font-family: " mce_style="font-size: 12pt; line-height: 115%; font-family: " lang="EN-US">This study shows that medicinal plants could be sources of compounds which can be used to fight against β-lactam resistant bacteria.</span></sp--> ER -