TY - JOUR AU - Oduor, Joseph Michael Ochieng' AU - Onkoba, Nyamongo AU - Maloba, Fredrick AU - Arodi, Washingtone Ouma AU - Nyachieo, Atunga PY - 2016/11/24 Y2 - 2024/03/28 TI - Efficacy of lytic Staphylococcus aureus bacteriophage against multidrug-resistant Staphylococcus aureus in mice JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 10 IS - 11 SE - Original Articles DO - 10.3855/jidc.7931 UR - https://jidc.org/index.php/journal/article/view/27886033 SP - 1208-1213 AB - <p class="SmallText">Introduction: The use of bacteriophages as an alternative treatment method against multidrug-resistant bacteria has not been explored in Kenya. This study sought to determine the efficacy of environmentally obtained lytic bacteriophage against multidrug-resistant <em>Staphylococcus aureus</em> (MDRSA) bacterium in mice.</p> <p class="SmallText">Methodology:<em> Staphylococcus aureus</em> bacterium and <em>S. aureus</em>-specific lytic phage were isolated from sewage and wastewater collected within Nairobi County, Kenya. Thirty mice were randomly assigned into three groups: MDRSA infection group (n = 20), phage-infection group (n = 5), and non-infection group (n = 5). The MDRSA infection group was further subdivided into three groups: clindamycin treatment (8 mg/kg; n = 5), lytic phage treatment (10<sup>8</sup> PFU/mL (n = 5), and a combination treatment of clindamycin and lytic phage (n = 5). Treatments were done at either 24 or 72 hours post-infection (p.i), and data on efficacy, bacterial load, and animal physical health were collected.</p> <p class="SmallText">Results: Treatment with phage was more effective (100%) than with clindamycin (62.25% at 24 hours p.i and 87.5% at 72 hours p.i.) or combination treatment (75% at 24 hours p.i. and 90% at 72 hours p.i.) (p &lt; 0.001).</p> <p class="SmallText">Conclusions: The results show that the environmentally obtained <em>S. aureus</em> lytic bacteriophage has therapeutic potential against MDRSA bacterium in mice.</p> ER -