TY - JOUR AU - Boonyanugomol, Wongwarut AU - Khuntikeo, Narong AU - Pugkhem, Ake AU - Sawadpanich, Kookwan AU - Hahnvajanawong, Chariya AU - Wongphutorn, Phattharaphon AU - Khampoosa, Bandit AU - Chomvarin, Chariya PY - 2017/01/30 Y2 - 2024/03/29 TI - Genetic characterization of Helicobacter pylori vacA and cagA genes in Thai gastro-duodenal and hepatobiliary patients JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 11 IS - 01 SE - Original Articles DO - 10.3855/jidc.8126 UR - https://jidc.org/index.php/journal/article/view/28141589 SP - 42-50 AB - <p class="SmallText">Introduction: <em>H. pylori</em> has been detected in patients with hepatobiliary diseases. It is currently unclear whether the <em>H. pylori</em> detected in hepatobiliary patients are genetically similar to those in gastro-duodenal patients. The aim of this study was to determine <em>H. pylori vac</em>A and <em>cag</em>A genotypes in Thai patients with gastro-duodenal and hepatobiliary diseases.</p> <p class="SmallText">Methodology: <em>H. pylori</em> DNA was extracted from samples from gastric biopsies of gastro-duodenal patients (n=100) and from bile samples of hepatobiliary patients (n=80). The <em>vac</em>A and <em>cag</em>A genotypes were performed via polymerase chain reaction (PCR) followed by DNA sequencing.</p> <p class="SmallText">Results: The <em>vac</em>A m1 was found in Thai hepatobiliary patients (90%) at a higher rate compared with gastro-duodenal patients (50%).The combined <em>vac</em>A s1a+c/m1 were mostly found in Thai gastro-duodenal and hepatobiliary patients. The <em>cag</em>A gene was detected in 94% of patients with gastro-duodenal diseases compared with 28.8% in those with hepatobiliary diseases (p&lt;0.05). On the other hand, the Western type <em>cag</em>A was more prominent among hepatobiliary patients (100%) than gastro-duodenal patients (57.4%), and this type was grouped into same cluster with Thai gastro-duodenal patients via phylogenetic analysis.</p> <p class="SmallText">Conclusions: Based on <em>vac</em>A and <em>cag</em>A analysis, we conclude that infection with <em>H. pylori</em> in gastro-duodenal and hepatobiliary patients may be caused by the different <em>H. pylori</em> strains.</p> ER -