TY - JOUR AU - Chakraborty, Pampi AU - Kulkarni, Savita AU - Rajan, Ramakrishna AU - Sainis, Krishna PY - 2018/01/10 Y2 - 2024/03/28 TI - Mycobacterium tuberculosis strains from ancient and modern lineages induce distinct patterns of immune responses JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 11 IS - 12 SE - Original Articles DO - 10.3855/jidc.8596 UR - https://jidc.org/index.php/journal/article/view/31626595 SP - 904-911 AB - <p>Introduction: It is possible that the difference in virulence and prevalence of different strains of <em>Mycobacterium tuberculosis</em> is related to the diverse immune response they evoke in the host. Outbreak strains have been shown to subvert the innate immune response as a potential host evasion mechanism. However, the immunological outcome of the interactions of different clinical strains with different host cells is still not understood.</p><p>Methodology: Extremely Drug Resistant (XDR) Beijing, a modern lineage clinical strain and a comparator ancient lineage strain, EAI-5, were selected for the present study. The early induction of proinflammatory cytokines in human peripheral blood monocyte derived macrophages (MDM), monocyte derived dendritic cells (MDDC) and whole blood (WB) infected by selected clinical isolates and laboratory strains H37Rv and BCG were assessed.</p><p>Results: The two clinical strains exhibited distinct patterns of cytokine induction. The ancient lineage strain induced substantially higher expression of all proinflammatory cytokines like TNF-α, IL-1β, IL-12 and chemokines like MCP-1, IL-8. However, the modern lineage strain exhibited suppressed response for the same. Further, the immune responses to two strains were conserved in infected MDM, MDDC and WB i.e. showing similar patterns of response across multiple human hosts. However, the differential response pattern was not observed when bacterial sonicates were used instead of live mycobacteria.</p><p>Conclusions: The lineage specific patterns in induction of proinflammatory cytokines and chemokines by different <em>M. tuberculosis</em> strains remain similar in macrophage and dendritic cells isolated from different individuals. The present study also confirms that whole cell sonicates of different lineages of <em>M. tuberculosis </em>fail to induce such lineage specific response.</p> ER -