TY - JOUR AU - Esmaeili Benvidi, Mozhgan AU - Houri, Hamidreza AU - Ghalavand, Zohreh AU - Nikmanesh, Bahram AU - Azimi, Hadi AU - Samadi, Roghayeh AU - Nodeh Farahani, Narges AU - Eslami, Gita PY - 2017/10/31 Y2 - 2024/03/28 TI - Toxin production and drug resistance profiles of pediatric methicillin-resistant Staphylococcus aureus isolates in Tehran JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 11 IS - 10 SE - Original Articles DO - 10.3855/jidc.9360 UR - https://jidc.org/index.php/journal/article/view/31600148 SP - 759-765 AB - <p>Introduction: <em>Staphylococcus aureus</em> is known to be a major cause of skin and soft tissue infections, pneumonia and invasive diseases. In this study, attempts were made to examine the prevalence of <em>tsst-1, eta, etb, </em>and<em> luk-PV </em>genes among methicillin-resistant <em>S. aureus</em> (MRSA) isolated from children in Tehran.</p><p>Methodology: In the present cross-sectional study, a total of 100 MRSA were isolated from children who were referred to a pediatric hospital during 11-month period of September 2014 to August 2015. Isolates were identified using biochemical tests and then, using PCR, the isolates were tested for the presence of <em>mecA</em>, <em>tsst-1, eta, etb, and luk-PV </em>genes. Susceptibility of isolates to cefoxitin, penicillin, erythromycin, clindamycin, gentamicin, rifampin, minocycline, co-trimoxazole, linezolid, and vancomycin were evaluated using standard methods.</p><p>Results: It was found that the MRSA isolates had the greatest resistance to clindamycin (72%) and erythromycin (59%), while the lowest rates of resistance were observed to be related to minocycline (6%) and rifampin (12%). All of isolates were sensitive to vancomycin and linezolid. The <em>mecA</em> gene was detected in all the isolates. Moreover, <em>luk-PV </em>and<em> tsst-1 </em>were detected in 18% and 17% of the isolates, respectively. None of the isolates harbored <em>eta</em> and <em>etb</em> genes.</p><p>Conclusions: Our data provide specifications about the toxin production status of <em>S. aureus</em> isolates from pediatric children. The current study showed increased resistance to different antibiotics in <em>S. aureus</em> isolates. Therefore, to prevent multi-resistance to other antibiotic classes, it is essential to withhold prescriptions and stop unessential use of available antibiotics.</p> ER -