TY - JOUR AU - El-Kafrawy, Sherif Aly AU - Jamjoom, Ghazi Abdulatif AU - Akbar, Hisham Othman AU - Fallatah, Hind Ibrahim Baker AU - El-Daly, Mai Mohamed AU - Qari, Yousef Abdulfattah AU - Alghamdi, Abdullah Saeed AU - Babatin, Mohamed AU - Alsaedi, Mohammed Abdullah AU - Othman, Noura Abdulhamid AU - Al-Subhi, Tagreed Lafi AU - Abdel-Hamid, Mohamed AU - Azhar, Esam Ibraheem PY - 2018/07/31 Y2 - 2024/03/28 TI - Analysis of Hepatitis B virus (HBV) mutations in patients from Western Saudi Arabia with chronic disease JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 12 IS - 07 SE - Original Articles DO - 10.3855/jidc.9534 UR - https://jidc.org/index.php/journal/article/view/31954005 SP - 557-567 AB - <p>Introduction: Extensive research has provided a link between HBV variants and the clinical complications of liver diseases. This study was performed to further investigate the relationship between HBV variants in preS, S and BCP/PC regions and disease progression in chronic hepatitis B (CHB) cases in Jeddah, Saudi Arabia.</p><p>Methodology: 182 CHB patients were recruited for this study. HBV DNA was amplified by PCR in the PreS, S, and BCP/PC regions. Sequences were generated from 31 and 26 treated cases in PreS and S regions respectively and from 72 cases in the BCP/PC region.</p><p>Results: The majority of cases (86.7%) were genotype D. Mutations at preS1-A2922C, X-A1624C and PC-G1887A were detected only in cases with either a high fibrosis score or hepatocellular carcinoma (HCC), while mutations at positions PC-C1982A, PC-G1951T, X-C1628T and X-A1630G were detected more frequently in HCC cases, without reaching statistical significance. Seven deletions were detected in the PreS-region. No deletions were detected in the CCAAT box.</p><p>The accumulation of mutations per sample in the preS1-2 and S regions were associated with elevated ALT (p &lt; 0.001, 0.001 and 0.001; respectively) and increased fibrosis (p = 0.018, 0.02 and 0.013; respectively). The accumulation of mutations per sample in the BCP/PC region is associated with high viral load. Occult hepatitis B infection (OBI) was identified in 5 samples.</p><p>Conclusion: Our results add to the knowledge about HBV genotype-D variants. The accumulation of mutations per sample and OBI seem to play a role in the progression of HBV infection. G1896A was associated with the HBeAg negativity. The preS deletions did not play a role in liver disease progression.</p> ER -