TY - JOUR AU - ElMahallawy, Hadir AU - Zafer, Mai Mahmoud AU - Al-Agamy, Mohamed AU - Amin, Magdy Aly AU - Mersal, Mai Muhammed AU - Booq, Rayan Yousef AU - Alyamani, Essam AU - Radwan, Samah PY - 2018/06/30 Y2 - 2024/03/28 TI - Dissemination of ST101 blaOXA-48 producing Klebsiella pneumoniae at tertiary care setting JF - The Journal of Infection in Developing Countries JA - J Infect Dev Ctries VL - 12 IS - 06 SE - Original Articles DO - 10.3855/jidc.9789 UR - https://jidc.org/index.php/journal/article/view/31940293 SP - 422-428 AB - <p>Introduction: The worldwide dissemination of the acquired carbapenemases in Gram-negative bacteria is a strongly expressed demand for the emergence of post antibiotic era. The aim of this study was to test the production of carbapenemase by <em>Klebsiella pneumoniae</em> strains isolated from hospitalized cancer patients and to investigate the genetic relationship of carbapenemase producing carbapenem resistant <em>K. pneumoniae</em> using multilocus sequence typing (MLST).</p><p>Methodology: Antibiotic susceptibility testing and phenotypic testing for extended spectrum b-lactamases (ESBL) and carbapenemases production were performed. PCR amplification of ESBL and carbapenemase genes was performed. MLST was done to detect the genetic relatedness of the isolates.</p><p>Results: Our data showed all strains were sensitive to colistin. Carba NP test was positive in thirty-one carbapenem resistant <em>K. pneumoniae</em> isolates and 26 out of 34 <em>K. pneumoniae </em>isolates were metallo-beta-lactamases (MBL) positive. All carbapenemase-positive isolates were ESBL CTX-M-1-like positive. <em>bla</em><sub>OXA-48</sub> gene was detected in 25 isolates (80.65%) and 21 isolates (67.75%) produced <em>bla</em><sub>NDM-1 </sub>like enzyme. VIM and KPC genes were not identified in this study. Association of <em>bla</em><sub>OXA-48 </sub>like and <em>bla</em><sub>NDM-1 </sub>like was found in 15 (48.39%) isolates, while the coproduction of OXA-48-like and IMP-1 was revealed in only one <em>K. pneumoniae</em> isolate. MLST revealed ten distinct sequence types (STs).</p><p>Conclusion: Here we have documented the coexistence of NDM-type and OXA-48-like, and the coproduction of OXA-48-like and IMP in carbapenem resistant <em>K. pneumoniae</em> in patients with cancer. The dominant clone of the OXA-48-like-producing<em> K. pneumoniae</em> isolates from Egypt was ST101 epidemic clone belonging to clonal complex 101, an association that has been reported worldwide. The second most frequent ST was ST383.ST11 was assigned to OXA-48-producing <em>K. pneumoniae</em>.</p> ER -