The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques – An age related comparative analysis

  • Ibrahim Kannosh University of Belgrade, Belgrade 11000, Serbia
  • Danijela Staletovic University of Pristina Kosovska Mitrovica, Kosovska Mitrovica 38220, Serbia
  • Bosko Toljic University of Belgrade, Belgrade 11000, Serbia
  • Milena Radunovic University of Belgrade, Belgrade 11000, Serbia
  • Ana Pucar University of Belgrade, Belgrade 11000, Serbia
  • Sanja Matic Petrovic University of Belgrade, Belgrade 11000, Serbia
  • Ivana Grubisa University of Belgrade, Belgrade 11000, Serbia
  • Milos Lazarevic University of Belgrade, Belgrade 11000, Serbia
  • Zlata Brkic University of Defense, Belgrade 11000, Serbia
  • Jelena Knezevic Vukcevic University of Belgrade, Belgrade 11000, Serbia
  • Jelena Milasin University of Belgrade, Belgrade 11000, Serbia
Keywords: periodontitis, atherosclerosis, periodontal microorganisms

Abstract

Introduction: There is a known connection between periodontitis and atherosclerosis and the presence of periopathogens in blood vessels. However, changes of the oral microflora related to the aging process and its possible effects on atherosclerosis, have yet to be analyzed. The aim of this study was to assess temporal changes in the frequency of periodontal bacteria in the subgingival plaque and in atherosclerotic blood vessels of patients with atherosclerosis.

Methodology:The study included 100 patients with atherosclerosis and periodontitis, divided into two groups, below and over 60 years of age. Clinical examinations were performedand subgingival plaque specimens were collected as well as biopsy specimens from the following arteries: coronary (34), carotid (29), abdominal (10), femoral (10), mammary (13) and iliac (4). Subgingival and artery specimens were subjected to PCR detection of 5 major periodontal pathogens: Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), Aggregatibacter actinomycetemcomitans (Aa), Tannerella forsythensis (Tf) and Treponema denticola (Td).

Results:Tf was the most and Td the least frequent bacteria in both age groups and in both types of samples. The frequencies of bacteria in subgingival versus atherosclerotic samples were: Tf (76%:53%), Pi (71%:31%), Pg (60%:38%), Aa (39%:14%) and Td (21%:6%). Only Aa and Pi showed a significant difference of prevalence between younger and older patients. The most colonized artery was a. coronaria, followed by a. carotis, a. abdominalis, a. mammaria, and a. femoralis.

Conclusions: Patient’s age and the distance of a given blood vessel from the oral cavity influenced microbiological findings in the atherotic plaque.

Author Biographies

Ibrahim Kannosh, University of Belgrade, Belgrade 11000, Serbia

Department of Human Genetics, School of Dental Medicine

Danijela Staletovic, University of Pristina Kosovska Mitrovica, Kosovska Mitrovica 38220, Serbia

Clinic for Dentistry, Faculty of Medicine

Bosko Toljic, University of Belgrade, Belgrade 11000, Serbia

Department of Human Genetics, School of Dental Medicine

Milena Radunovic, University of Belgrade, Belgrade 11000, Serbia

Department of Microbiology and Immunology, School of Dental Medicine

Ana Pucar, University of Belgrade, Belgrade 11000, Serbia

Department of Periodontology and Oral Medicine, School of Dental Medicine

Sanja Matic Petrovic, University of Belgrade, Belgrade 11000, Serbia

Department of Periodontology and Oral Medicine, School of Dental Medicine

Ivana Grubisa, University of Belgrade, Belgrade 11000, Serbia

Department of Human Genetics and Prenatal Diagnostics, Zvezdara, University Medical Center

Milos Lazarevic, University of Belgrade, Belgrade 11000, Serbia

Department of Human Genetics, School of Dental Medicine

Zlata Brkic, University of Defense, Belgrade 11000, Serbia

Clinic of Dental Medicine, Faculty of Medicine of the Military Medical Academy

Jelena Knezevic Vukcevic, University of Belgrade, Belgrade 11000, Serbia

Laboratory of Microbiology, Faculty of Biology

Jelena Milasin, University of Belgrade, Belgrade 11000, Serbia

Department of Human Genetics, School of Dental Medicine

Published
2018-12-31
How to Cite
1.
Kannosh I, Staletovic D, Toljic B, Radunovic M, Pucar A, Matic Petrovic S, Grubisa I, Lazarevic M, Brkic Z, Knezevic Vukcevic J, Milasin J (2018) The presence of periopathogenic bacteria in subgingival and atherosclerotic plaques – An age related comparative analysis. J Infect Dev Ctries 12:1088-1095. doi: https://doi.org/10.3855/jidc.10980
Section
Original Articles