Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance

  • Moustafa Abdel Fadeel US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
  • Brent L House US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
  • Momtaz M Wasfy US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
  • John D Klena US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
  • Engy E Habashy US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
  • Mayar M Said US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
  • Mohamed Abdel Maksoud US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
  • Bassem Abdel Rahman US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt
  • Guillermo Pimentel US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Abstract

Introduction: Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations.

Methodology: Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses a mixture of somatic and flagellar antigens to detect the total antibody response against Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) infection. The levels of Ig isotype response (IgG, IgM and IgA) were also evaluated, and results were compared to those of TUBEX-TF and Typhidot commercial kits. 

Results: Of 234 culture-confirmed typhoid patients, the total Ig ELISA diagnosed 93% compared to 71% using Widal test. This sensitivity level (93%) is higher than that observed for the individual Ig ELISAs (IgG 75%; IgM 79%; IgA 57%) and the commercial tests TUBEX-TF (75%), Typhidot IgM (63%) and Typhidot IgG (28%). An agreement of 78% was achieved between the total Ig ELISA and Widal test. The average specificity of the ELISA was 96%. Using ELISA, up to 200 samples can be tested per run with cost per test at US$0.20.

Conclusions: The developed ELISA shows superior sensitivity and specificity, when compared to Widal, TUBEX-TF and Typhidot assays, is more cost effective and allows higher throughput. This method is highly recommended for active surveillance studies or outbreak investigations of typhoid fever.

Author Biographies

Moustafa Abdel Fadeel, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research scientist

Head, Serology/LUMINEX Section, GDDRP Lab,

Brent L House, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research scientist

Head, GDDRP Lab Unit

Momtaz M Wasfy, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research scientist

Head, GDDRP Lab Unit

John D Klena, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research scientist

Head, Clinical trials and Millitary studies (CTMS).

Engy E Habashy, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research technician

GDDRP Lab Unit

Mayar M Said, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research technologist

GDDRP Lab Unit

Mohamed Abdel Maksoud, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research technician

GDDRP Lab Unit

Bassem Abdel Rahman, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research technician

GDDRP Lab Unit

Guillermo Pimentel, US Naval Medical Research Unit No. 3 (NAMRU-3), Cairo, Egypt

Research scientist

Deputy Head, Virology and Zoontic Diseases Research Program

Published
2011-03-21
How to Cite
Fadeel M, House B, Wasfy M, Klena J, Habashy E, Said M, Maksoud M, Rahman B, Pimentel G (2011) Evaluation of a newly developed ELISA against Widal, TUBEX-TF and Typhidot for typhoid fever surveillance. The Journal Of Infection In Developing Countries 5 (03): 169-175. https://doi.org/10.3855/jidc.1339
Section
Original Articles

Keywords

typhoid; serological diagnosis; ELISA; surveillance