Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices

Authors

  • Maicon Henrique Caetano Universidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas (Ibilce), Campus São José do Rio Preto, São Paulo, Brazil
  • Adriano Menis Ferreira Universidade Federal de Mato Grosso do Sul, Três Lagoas, Mato Grosso do Sul, Brazil
  • Denise de Andrade Human Exposome and Infectious Diseases Network (HEID); Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil
  • Álvaro Francisco Lopes de Sousa Human Exposome and Infectious Diseases Network (HEID); Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil
  • Liliane Moretti Carneiro Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil
  • Alessandra Lyrio Barbosa Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil
  • Herica Emilia Félix de Carvalho Human Exposome and Infectious Diseases Network (HEID); Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, São Paulo, Brazil
  • Marcelo Alessandro Rigotti Universidade Federal de Mato Grosso do Sul, Três Lagoas, Mato Grosso do Sul, Brazil
  • Margarete Teresa Gottardo de Almeida Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil

DOI:

https://doi.org/10.3855/jidc.15344

Keywords:

Ozone, ozonation, Candida albicans, Aspergillus fumigatus, disinfection

Abstract

Introduction: Candida albicans and Aspergillus fumigatus are two important agents of Healthcare-associated infections. This study aimed to evaluate the antifungal activity of ozone (O3) gas produced by two commercial devices against cultures of these two species.

Methodology: Sterile plastic plates were inoculated with C. albicans and A. fumigatus and placed on a countertop at three distances (30 cm, 1 m, and 2 m) and three positions in relation to the wall (near, middle, and away), considering the source of O3. Plates were exposed to O3 for one hour and incubated. After incubation, the counting of colony-forming units was performed. As a control, an inoculated plate was incubated, without being exposed to O3. Tests were carried out with two different devices (namely, Mod.I and Mod.II), with the air conditioner on and off, in triplicate.

Results: Both devices showed antifungal activity. Mod. I presented better results, due to a higher flow rate. The best activity was on plates at 30 cm, middle position. Contrarily, on plates at 2m, near the wall, the inhibition activity was lower. The best results were obtained with the air conditioner off. Candida albicans was more sensitive to O3 than A. fumigatus.

Conclusions: This method of decontamination by O3 gas shows potential due to its fast and easy execution. The establishment of new protocols for hygiene and hospital disinfection using this approach should be considered, which may reduce environmental contamination by fungi and, consequently, the burden of fungal infections.

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Published

2024-03-31

How to Cite

1.
Caetano MH, Menis Ferreira A, de Andrade D, Lopes de Sousa Álvaro F, Moretti Carneiro L, Lyrio Barbosa A, Félix de Carvalho HE, Rigotti MA, Gottardo de Almeida MT (2024) Effect of ozone gas on cultures of Candida albicans and Aspergillus fumigatus: evaluation of two ozonation devices. J Infect Dev Ctries 18:473–479. doi: 10.3855/jidc.15344

Issue

Vol. 18 No. 03: March 2024

Section

Original Articles

License

Copyright (c) 2024 Maicon Henrique Caetano, Adriano Menis Ferreira, Denise de Andrade, Álvaro Francisco Lopes de Souza, Liliane Moretti Carneiro, Alessandra Lyrio Barbosa, Herica Emília Félix de Carvalho, Marcelo Alessandro Rigotti, Margarete Teresa Gottardo de Almeida

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