Prevalence of carbapenemases among Gram-negative bacteria in Tunisia: first report of KPC-2 producing Acinetobacter baumannii

Authors

  • Dhouha Miniaoui Microbes Evolution Phylogenie et Infections (MEPHI), Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, 13385 Marseille Cedex 05, France https://orcid.org/0009-0008-1597-5069
  • Olfa Dziri Université de Carthage, Laboratoire de Recherche des Sciences et Technologies de l’Environnement, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Technopôle de Borj-Cedria, BP-1003, Hammam-Lif 2050, Tunisie
  • Yomna Ben Lamine Université Tunis El Manar, Laboratoire de Biologie Clinique, Institut Mohamed Kassab d’Orthopédie, Rue des travailleurs Ksar Saïd La Mannouba 2010, Tunis. Tunisie
  • Allaaeddin A El Salabi Department of Environmental Health, Faculty of Public Health, University of Benghazi, Benghazi, Libya https://orcid.org/0000-0001-7739-0543
  • Elham O Omar Department of Medical Microbiology, Faculty of Medicine, University of Benghazi, Benghazi, Libya https://orcid.org/0009-0002-6619-2095
  • Khouloud Slimene Microbes Evolution Phylogenie et Infections (MEPHI), Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, 13385 Marseille Cedex 05, France
  • Raoudha Dziri Université de Tunis El-Manar, Laboratoire des Microorganismes et Biomolécules Actives. Faculté des Sciences de Tunis. Campus Universitaire, 2098 El-Manar II, Tunisie https://orcid.org/0000-0003-1783-5672
  • Sophia Bouhalila-Besbes Université Tunis El Manar, Laboratoire de Biologie Clinique, Institut Mohamed Kassab d’Orthopédie, Rue des travailleurs Ksar Saïd La Mannouba 2010, Tunis. Tunisie
  • Linda Hadjadj Microbes Evolution Phylogenie et Infections (MEPHI), Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, 13385 Marseille Cedex 05, France
  • Aymen Mabrouk Université Tunis El Manar, Faculté de Médecine de Tunis, LR18ES39, Centre National de Greffe de Moelle Osseuse 1006, Tunis, Tunisie
  • Ahmed I Elbousify Department of Pediatric, Faculty of Medicine, Tripoli University, Tripoli, Libya
  • Seydina M Diene Microbes Evolution Phylogenie et Infections (MEPHI), Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, 13385 Marseille Cedex 05, France https://orcid.org/0000-0002-6466-2324
  • Jean-Marc Rolain Microbes Evolution Phylogenie et Infections (MEPHI), Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, 13385 Marseille Cedex 05, France
  • Chedly Chouchani niversité de Carthage, Laboratoire de Recherche des Sciences et Technologies de l’Environnement, Institut Supérieur des Sciences et Technologies de l’Environnement de Borj-Cedria, Technopôle de Borj-Cedria, BP-1003, Hammam-Lif 2050, Tunisie

DOI:

https://doi.org/10.3855/jidc.17978

Keywords:

Gram-negative bacteria, OXA-23, NDM-1, VIM-2, KPC-2, Tunisia

Abstract

Introduction: The rapid evolution of the antibacterial resistance problem worldwide, including the Mediterranean countries, constitutes a real threat to public health. This study aims to characterize carbapenemase encoding genes among Gram-negative bacteria collected from some Tunisian hospitals.

Methodology: Twenty-two clinical carbapenem-resistant Gram-negative bacteria were recovered, and identified by the matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) method. Antibiotic resistance was tested by disk diffusion method on Muller-Hinton Agar. The minimum inhibitory concentration (MIC) for imipenem was revealed by the E-test method. Carbapenemase encoding genes were screened by polymerase chain reaction (PCR). Genetic relatedness was performed by the pulsed field gel electrophoresis (PFGE) method.

Results: Our isolates, identified as K. pneumoniae (n = 7), P. mirabilis (n = 1), A. baumannii (n = 13), and P. aeruginosa (n = 1), presented high MIC values for imipenem. Enterobacerales were resistant to carbapenems due to OXA-48 production. Only, four K. pneumoniae harbored the blaNDM-1 gene. VIM-2 production was detected in P. aeruginosa. However, OXA-23 production was observed in A. baumannii isolates, one of which co-produced the KPC-2 enzyme that was identified for the first time in Tunisia in this species. A high genetic diversity was demonstrated by pulsed-field gel electrophoresis in K. pneumoniae and A. baumannii after XbaI and ApaI digestion respectively.

Conclusions: Our findings highlight the spread of various unrelated clones of carbapenemase-producers in some Tunisian hospitals as well as the spread of several carbapenemase types.

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Published

2023-11-30

How to Cite

1.
Miniaoui D, Dziri O, Ben Lamine Y, El Salabi AA, Omar EO, Slimene K, Dziri R, Bouhalila-Besbes S, Hadjadj L, Mabrouk A, Elbousify AI, Diene SM, Rolain J-M, Chouchani C (2023) Prevalence of carbapenemases among Gram-negative bacteria in Tunisia: first report of KPC-2 producing Acinetobacter baumannii. J Infect Dev Ctries 17:1591–1597. doi: 10.3855/jidc.17978

Issue

Vol. 17 No. 11: November 2023

Section

Original Articles

License

Copyright (c) 2023 Dhouha Miniaoui, Olfa Dziri, Yomna Ben Lamine, Allaaeddin A El Salabi, Elham O Omar, Khouloud Slimene, Raoudha Dziri, Sophia Bouhalila-Besbes, Linda Hadjadj, Aymen Mabrouk, Ahmed I Elbousify, Seydina M Diene, Jean-Marc Rolain, Chedly Chouchani

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