Amplification of mecA gene in multi-drug resistant Staphylococcus aureus strains from hospital personnel

  • Asad U. Khan Interdisciplinary Biotechnology Unit, Aligarh Muslim University Aligarh 202002
  • Ayesha Sultan Interdisciplinary Biotechnology Unit, Aligarh Muslim University Aligarh 202002
  • Anju Tyagi Microbiology Department JNMC, AMU, Aligarh
  • Shazia Zahoor Gastroenterology Department, PGIMER, Chandigarh
  • Mohd Akram Interdisciplinary Biotechnology Unit, Aligarh Muslim University Aligarh 202002
  • Sukhminderjit Kaur Gastroenterology Department, PGIMER, Chandigarh
  • Mohd Shahid Microbiology Department JNMC, AMU, Aligarh
  • Chetana V. Vaishnavi affi
Keywords: methicillin resistance, Staphylococcus aureus, plasmid, mecA gene, hospital personnel

Abstract

Background: Antibiotic resistance is common among bacterial pathogens associated with both community acquired and nosocomial infections. In view of the present problem of drug resistance we investigated the prevalence of methicillin resistant Staphylococcus aureus (MRSA) and amplified the mecA gene in the isolates from the hand swabs of the hospital personnel. Methodology: The nuc gene was amplified to characterize these isolates at species level. The S. aureus isolates were analyzed for their susceptibility to different classes of antibiotics using the disk diffusion method. The spot inoculation test was performed to detect methicillinase production in these isolates. Results: In the screened isolates of S. aureus, 14.2 and 15 kb of plasmids were present. These isolates showed pronounced resistance against β-lactam antibiotics including second- and third-generation cephalosporins, aminoglycosides, macrolides and fluoroquinolone. Some of the isolates included in this study were resistant to three or more antibiotics. Expression of methicillinase was detected by spot inoculation test, and a few of the isolates were found to produce methicillinase. Moreover, mecA gene was also amplified. Of 17 isolates only 7 showed presence of mecA gene. Conclusion: This study highlights the emerging trend of multiple drug resistance in S. aureus strains isolated from hospital personnel working in a premier hospital in North India.
Published
2007-12-01
How to Cite
1.
Khan AU, Sultan A, Tyagi A, Zahoor S, Akram M, Kaur S, Shahid M, Vaishnavi CV (2007) Amplification of mecA gene in multi-drug resistant Staphylococcus aureus strains from hospital personnel. J Infect Dev Ctries 1:289-295. doi: 10.3855/jidc.366
Section
Original Articles