Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus

Authors

  • Riza Adaleti Clinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, Istanbul
  • Yasar Nakipoglu Department of Microbiology and Clinical Microbiology, Faculty of Medicine, University of Istanbul, Istanbul
  • Zeynep Ceren Karahan Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Ankara University, Ankara
  • Cihan Tasdemir Clinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, Istanbul
  • Fatma Kaya Clinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, Istanbul

DOI:

https://doi.org/10.3855/jidc.321

Keywords:

Methicillin-resistant Staphylococcus aureus (MRSA), polymerase chain reaction (PCR), oxacillin disk diffusion, cefoxitin disk diffusion, oxacillin agar screening

Abstract

Background: Accurate and rapid detection of methicillin-resistant Staphylococcus aureus is very important in a clinical laboratory setting to avoid treatment failure. Conventional methods were compared against the gold standard polymerase chain reaction (PCR) technique to determine the best combination of the routine procedures. Methodology: Methicillin resistance was investigated in 416 clinical Staphylococcus aureus isolates by PCR, oxacillin agar screening (OAS), oxacillin disk diffusion (ODD) and cefoxitin disk diffusion (CDD) methods. Results: Two hundred and ten (51%) out of 416 S. aureus strains were found to be mecA-positive by PCR. Sensitivity and specificity of the ODD, CDD and OAS methods were detected as follows: 100% and 89%, 99.50% and 100%, and 99.50% and 100%, respectively. Conclusion: Combining the ODD and CDD methods could be a good choice for detecting methicillin resistance in S. aureus strains where mecA PCR cannot be performed.

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Published

2008-02-01

How to Cite

1.
Adaleti R, Nakipoglu Y, Karahan ZC, Tasdemir C, Kaya F (2008) Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus. J Infect Dev Ctries 2:046–050. doi: 10.3855/jidc.321

Issue

Section

Original Articles