Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus

Riza Adaleti; Yasar Nakipoglu; Zeynep Ceren Karahan; Cihan Tasdemir; Fatma Kaya

doi:10.3855/jidc.321

Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus

Authors

Riza Adaleti Clinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, Istanbul
Yasar Nakipoglu Department of Microbiology and Clinical Microbiology, Faculty of Medicine, University of Istanbul, Istanbul
Zeynep Ceren Karahan Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Ankara University, Ankara
Cihan Tasdemir Clinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, Istanbul
Fatma Kaya Clinical Microbiology Laboratory, Haydarpasa Numune Education and Research Hospital, Istanbul

DOI:

https://doi.org/10.3855/jidc.321

Keywords:

Methicillin-resistant Staphylococcus aureus (MRSA), polymerase chain reaction (PCR), oxacillin disk diffusion, cefoxitin disk diffusion, oxacillin agar screening

Abstract

Background: Accurate and rapid detection of methicillin-resistant Staphylococcus aureus is very important in a clinical laboratory setting to avoid treatment failure. Conventional methods were compared against the gold standard polymerase chain reaction (PCR) technique to determine the best combination of the routine procedures. Methodology: Methicillin resistance was investigated in 416 clinical Staphylococcus aureus isolates by PCR, oxacillin agar screening (OAS), oxacillin disk diffusion (ODD) and cefoxitin disk diffusion (CDD) methods. Results: Two hundred and ten (51%) out of 416 S. aureus strains were found to be mecA-positive by PCR. Sensitivity and specificity of the ODD, CDD and OAS methods were detected as follows: 100% and 89%, 99.50% and 100%, and 99.50% and 100%, respectively. Conclusion: Combining the ODD and CDD methods could be a good choice for detecting methicillin resistance in S. aureus strains where mecA PCR cannot be performed.

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Published

2008-02-01

How to Cite

Adaleti R, Nakipoglu Y, Karahan ZC, Tasdemir C, Kaya F (2008) Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus. J Infect Dev Ctries 2:046–050. doi: 10.3855/jidc.321

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Issue

Vol. 2 No. 01: February 2008

Section

Original Articles

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