Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Sanae Ammari; Amin Laglaoui; Latifa En-nanei; Sophie Bertrand; Christa Wildemauwe; Mohammed Abid

doi:10.3855/jidc.617

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Authors

Sanae Ammari Service de recherche, Laboratoire de biologie moléculaire, Institut Pasteur du Maroc, Tanger
Amin Laglaoui Equipe de Recherche sur la Valorisation Biotechnologique des Microorganisme ERVABIM, Faculté des Sciences et Techniques, B.P. 416, Tanger
Latifa En-nanei Service de recherche, Laboratoire de biologie moléculaire, Institut Pasteur du Maroc, Tanger
Sophie Bertrand Bacteriology Division, Scientific Institute of Public Health, J. Wytsman Street, 14 B-1050, Brusels
Christa Wildemauwe Pasteur Institute, Phage typing service, Engelandstreet 642- B-1180, Brussels
Mohammed Abid Service de recherche, Laboratoire de biologie moléculaire, Institut Pasteur du Maroc, Tanger

DOI:

https://doi.org/10.3855/jidc.617

Keywords:

Salmonella Enteritidis, ERIC-PCR, phage typing, PFGE, plasmid, antimicrobial resistance

Abstract

Objectives: This study aimed to investigate Salmonella Enteritidis strains isolated from human and food sources in the north of Morocco by means of phenotypic and genotypic methods.

Methodology: Fifteen isolates from humans and food were submitted to phage typing, XbaI-macrorestriction (pulsed field gel electrophoresis [PFGE]), enterobacterial repetitive intergenic consensus (ERIC-PCR), antimicrobial susceptibility testing, and PCR assay targeting the spvR and invA genes.

Results: Six fingerprinting profiles were obtained with the ERIC-PCR method, four with PFGE profiling, five with antimicrobial resistance, three with phage typing, and only one with plasmid profiling. spvR gene was detected in six strains, which did not harbour plasmids of 90 kb.

Conclusions: The conclusions of this study are drawn from a limited number of isolates. It would be desirable to investigate a greater and more diverse population of Salmonella isolates. S. Enteritidis was genotyped and showed four different patterns by PFGE and six by ERIC-PCR. Accordingly, high genetic similarity and limited genetic diversity were found for these strains from north of Morocco.

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How to Cite

Ammari S, Laglaoui A, En-nanei L, Bertrand S, Wildemauwe C, Abid M (2009) Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco. J Infect Dev Ctries 3:695–703. doi: 10.3855/jidc.617

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Issue

Vol. 3 No. 09: October 2009

Section

Original Articles

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