First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt.

  • Sameh M.M. AbdelGhani Cairo University School of Pharmacy
  • Ellen Smith Moland Creighton University School of Medicine
  • Jennifer A. Black Creighton University School of Medicine
  • Nancy D. Hanson Creighton University School of Medicine
  • Richard V. Goering Creighton University School of Medicine
  • Magdy A. Amine Cairo University School of Pharmacy
  • Amal E. Saafan Beni-Seif University School of Pharmacy
  • Maha Gaafar Cairo University School of Medicine
  • Mariam Younan Cairo University School of Medicine
  • Kenneth S. Thomson Creighton University School of Medicine
Keywords: CTX-M ESBLs, Enterobacteriaceae, Egypt

Abstract

Three pediatric isolates of Salmonella serovar Typhimurium from Egypt were reported as
CTX-M-14 ESBL producers. Two of the 3 isolates (68 and 111) were 85% related by PFGE;however both were distinct from isolate 94. All three isolates produced multiple clavulanate sensitive β-lactamase bands on IEF with pIs of 7.9 and 5.4 (all isolates), while pI 8.2 enzyme was produced by isolate 94 and pI 7.4 enzyme by isolate 111. The pI 7.9 enzyme was transferable by conjugation and conferred a CTX-M ESBL phenotype. PCR and sequencing identified the CTX-M gene as CTX-M-14. Genes for OXA-1 and SHV-12 were also identified by sequencing. To our knowledge this is the first report of CTX-M-producing Salmonella in Egypt.

Author Biographies

Sameh M.M. AbdelGhani, Cairo University School of Pharmacy
Assitant Lecturer , Microbiology and Immunology Department Faculty of pharmacy , Beni-Seif University Egypt.
Nancy D. Hanson, Creighton University School of Medicine
Ph.D.
Richard V. Goering, Creighton University School of Medicine
Ph.D.
Magdy A. Amine, Cairo University School of Pharmacy
Ph.D.
Amal E. Saafan, Beni-Seif University School of Pharmacy
Ph.D.
Maha Gaafar, Cairo University School of Medicine
Ph.D.
Mariam Younan, Cairo University School of Medicine
Ph.D.
Kenneth S. Thomson, Creighton University School of Medicine
Ph.D.
Published
2009-12-14
How to Cite
1.
AbdelGhani SM, Moland ES, Black JA, Hanson ND, Goering RV, Amine MA, Saafan AE, Gaafar M, Younan M, Thomson KS (2009) First Report of CTX-M-14 Producing Clinical Isolates of Salmonella serovar Typhimurium from Egypt. J Infect Dev Ctries 4:058-060. doi: 10.3855/jidc.386
Section
Letters to the Editor