Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains

  • Rosina Khan Aligarh Muslim University, Aligarh, India
  • Mohammad Zakir Aligarh Muslim University, Aligarh, India
  • Sadul H Afaq Aligarh Muslim University, Aligarh, India
  • Abdul Latif Aligarh Muslim University, Aligarh, India
  • Asad U Khan Aligarh Muslim University, Aligarh, India
Keywords: Multidrug resistance, ESBLs, plant extracts, bacteria, fungus

Abstract

Background: The emerging trends of multidrug resistance among several groups of microorganisms against different classes of antibiotics led different researchers to develop efficient drugs from plant sources to counter multidrug resistant strains. This study investigated different solvent extracts of Prosopis spicigera (P. Spicigera), Zingiber officinale, and Trachyspermum ammi (T. ammi) to determine their efficacy against multidrug resistant microbes.

Methodology: Successive extractions of these plants were performed using a Soxhlet apparatus, using solvents with increasing polarities. Preliminary phytochemical analysis was also performed .Minimum inhibitory concentration was determined by a two-fold serial dilution method followed by determination of minimum bactericidal/fungicidal concentration. Multidrug resistant (MDR) strains of Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, Escherichia coli and reference strains of Streptococcus mutans and Streptococcus bovis were used in the study.

Results: The ethanolic fraction of P. spicigera (least minimum inhibitory concentration [MIC] - 4.88 µg/ml) demonstrated a remarkable inhibition of the microorganisms while fractions obtained from those of Zingiber officinale (least MIC-78.125 µg/ml) exhibited little activity. The petroleum ether fraction of T. ammi (least MIC- 625 µg/ml) showed best activity when compared to its other fractions. Qualitative analysis of the phytoconstituents was also performed.

Conclusions: The potency shown by these extracts recommends their use against multidrug resistant microorganisms. This study also showed that P. spicigera could be a potential source of new antimicrobial agents.

Author Biographies

Rosina Khan, Aligarh Muslim University, Aligarh, India
Interdiaciplinary BIotechnology Unit
Mohammad Zakir, Aligarh Muslim University, Aligarh, India
Interdiaciplinary BIotechnology Unit
Sadul H Afaq, Aligarh Muslim University, Aligarh, India
Pharmacology Departmnet
Abdul Latif, Aligarh Muslim University, Aligarh, India
Pharmacollogy Department
Asad U Khan, Aligarh Muslim University, Aligarh, India
Interdiaciplinary BIotechnology Unit
Published
2010-05-09
How to Cite
1.
Khan R, Zakir M, Afaq SH, Latif A, Khan AU (2010) Activity of solvent extracts of Prosopis spicigera, Zingiber officinale and Trachyspermum ammi against multidrug resistant bacterial and fungal strains. J Infect Dev Ctries 4:292-300. doi: 10.3855/jidc.621
Section
Original Articles