First report of a Tn402-like class 1 integron carrying blaVIM-2 in Pseudomonas putida from Argentina

Authors

  • Patricia Marchiaro Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario
  • Alejandro M Viale Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario
  • Viviana Ballerini Servicio de Bacteriología, Hospital Intendente Carrasco, Secretaría de Salud Pública, Municipalidad de Rosario
  • Gustavo Rossignol Servicio de Bacteriología, Hospital Provincial de Rosario
  • Alejandro J Vila Departamento de Química Biológica, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario
  • Adriana Limansky Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

DOI:

https://doi.org/10.3855/jidc.1012

Keywords:

Pseudomonas putida, class 1 integron, Tn402-like transposon, blaVIM-2, metallo-B-lactamases

Abstract

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Author Biographies

Patricia Marchiaro, Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

Patricia M. Marchiaro, borned 1969 in Rosario, Santa Fe, Argentina, obtained a degree on Biochemistry at the School of Biochemistry and Pharmacy of the National University of Rosario, and a Master in Clinical Bacteriology of the National University of Rosario. She is currently a Fellow of Universidad Nacional de Rosario, working for her doctoral degree at the Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Departament of Microbiology, Facultad de Ciencias Bioquímicas y Farmacéuticas, Rosario, Argentina. Her subject of study involves: “Epidemiology of antimicrobial bacterial resistance mechanisms”. Some of her last publications are:

- Quinones-Falconi, F., Galicia-Velasco, M., Marchiaro, P., Mussi, M.A., Ballerini, V., Vila, A. J., Viale, A. M. Bermejo-Morales, K., Limansky, A.S. Emergence of Pseudomonas aeruginosa Strains Producing Metallo-bata-lactamases of the IMP-15 and VIM-2 types in Mexico. Clinical Microbiology and Infection 16:126-131 (2010).

- Marchiaro, P., Ballerini, V., Spalding, T., Cera, G., Mussi, M.A., Morán Barrio, J., Vila, A.J., Viale, A.M., and Limansky, A.S. A convenient microbiological assay employing cell-free extracts for the rapid characterization of Gram-negative carbapenemase producers. Journal of Antimicrobial Chemotherapy 62: 336-344 (2008).

- Marchiaro, P., Tomatis, P., Mussi, M.A., Pasterán, F., Limansky, A.S., Viale, A.M., Vila, A.J. Biochemical characterization of metallo-β-lactamase VIM-11 from a Pseudomonas aeruginosa clinical strain. Antimicrobial Agents and Chemotherapy 52: 2250-2252 (2008).

-Marchiaro, P., Mussi, M.A., Ballerini, V., Pasteran, F., Viale, A.M., Vila, A.J., and Limansky, A.S. Sensitive EDTA-Based Microbiological Assays for the Detection of Metallo-β-lactamases in Non-Fermentative Gram-Negative Bacteria. J Clin Microbiol 43: 5648-5352 (2005).

Alejandro M Viale, Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

Alejandro M. Viale, borned 1953 in Rosario, Argentina, obtained a Doctorate degree in Biochemistry at the National University of Rosario and is currently Associate Professor of Microbiology at the Departament of Microbiology, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina. He is Principal Investigator of the Argentine Research Council (CONICET) working at the Instituto de Biología Molecular y Celular de Rosario (IBR). His area of research involves: “Antimicrobial bacterial resistance and molecular chaperones”. Some of his last publications are:

-Morán-Barrio J, Limansky AS, Viale AM. Secretion of GOB metallo-beta-lactamase in Escherichia coli depends strictly on the cooperation between the cytoplasmic DnaK chaperone system and the Sec machinery: completion of folding and Zn(II) ion acquisition occur in the bacterial periplasm. Antimicrob Agents Chemother. 53: 2908-2917 (2009).

-Marchiaro, P., Tomatis, P., Mussi, M.A., Pasterán, F., Limansky, A.S., Viale, A.M., Vila, A.J. (2008) Biochemical characterization of metallo-β-lactamase VIM-11 from a Pseudomonas aeruginosa clinical strain. Antimicrob Agents and Chemotherapy 52: 2250-2252 (2008).

- Mussi, M.A., Relling, V., Limansky, A. and Viale A. CarO, an Acinetobacter baumannii outer membrane protein involved in carbapenem resistance, is essential for L-ornithine uptake. FEBS Lett. 581: 5573-5578 (2007).

- Mussi, M.A., Limansky, A. and Viale A. Acquisition of Resistance to Carbapenems in Multidrug-Resistant Clinical Strains of Acinetobacter baumannii: Natural insertional inactivation of a gene encoding a member of a novel family of beta-barrel outer membrane proteins. Antimicrob Agents Chemot 49: 1432-1440 (2005).

Viviana Ballerini, Servicio de Bacteriología, Hospital Intendente Carrasco, Secretaría de Salud Pública, Municipalidad de Rosario

Viviana Ballerini, borned 1959 in Rosario, Santa Fe, Argentina, obtained a degree on Biochemistry at the School of Biochemistry and Pharmacy of the National University of Rosario. She is currently Chief of Staff of the Bacteriological Section of the Hospital Intendente Carrasco, Departamento Bioquímico Municipal, Secretaría de Salud Pública, Municipalidad de Rosario. She is conducting joint efforts with the above investigators composing a research team devoted to the study of the “Epidemiology of antimicrobial bacterial resistance mechanisms”. Some of her last publications are listed above.

Gustavo Rossignol, Servicio de Bacteriología, Hospital Provincial de Rosario

Gustavo Rossignol, borned 1966 in Rosario, Santa Fe, Argentina, obtained a degree on Biochemistry at the School of Biochemistry and Pharmacy of the National University of Rosario. He is currently Member of the staff of the Bacteriological Section of the Hospital Provincial de Rosario. His last publication is:

-Limansky, A. S. Zamboni, M.I., Guardati, M.C., Rossignol, G., Campos, E., Viale, A.M. Evaluation of phenotypic and genotypic markers for clinical strains of Acinetobacter baumannii. Medicina, 64: 306-312 (2004).

Alejandro J Vila, Departamento de Química Biológica, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

Alejandro J. Vila, borned 1962 in Rosario, Argentina, obtained a Doctorate degree in Chemistry at the University of Rosario and is currently Professor of Biophysics in the Departament of Biological Chemistry, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina. He is Principal Investigator of the Argentine Research Council (CONICET) working at the Instituto de Biología Molecular y Celular de Rosario (IBR). His area of research involves: “Evolution, Diversity, and Catalytic Mechanism of Metallo-B-Lactamases”. Some of his last publications are:

- Lisa MN, Hemmingsen L, Vila AJ. Catalytic role of the metal ion in the metallo-beta-lactamase GOB. J Biol Chem. 2010 12, 285(7):4570-7.

- Abriata LA, Ledesma GN, Pierattelli R, Vila AJ. Electronic structure of the ground and excited states of the Cu(A) site by NMR spectroscopy. J Am Chem Soc 131:1939-46 (2009).

-Tomatis PE, Fabiane SM, Simona F, Carloni P, Sutton BJ and Vila AJ. Adaptive protein evolution grants organismal fitness by improving catalysis and flexibility. Proc Natl Acad Sci USA, 105: 20605-20610 (2008).

-Llarrull LI, Tioni MF and Vila AJ. Metal Content and Localization during Turnover in B.cereus Metallo-B-Lactamase. J Am Chem Soc 130: 15842-15851 (2008).

-Morán-Barrio J, González JM, Lisa MN, Costello AL, Dal Peraro M, Carloni P, Bennett B, Tierney DL, Limansky AS, Viale AM and Vila AJ. The Metallo-beta-lactamase GOB is a mono-Zn(II) enzyme with a novel active site. J Biol Chem 282: 18286-93 (2007).

Adriana Limansky, Departamento de Microbiología, Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario

Adriana S. Limansky, borned 1962 in Rafaela, Santa Fe, Argentina, obtained a Doctorate degree in Biological Sciences at the National University of Rosario and is currently Assistant Professor of Clinical Bacteriology at the Departament of Microbiology, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina. She is an Independent Researcher at the Instituto de Biología Molecular y Celular de Rosario (IBR, CONICET). Her area of research involves: “Epidemiology of antimicrobial bacterial resistance mechanisms”. Some of her last publications are:

- Quinones-Falconi, F., Galicia-Velasco, M., Marchiaro, P., Mussi, M.A., Ballerini, V., Vila, A. J., Viale, A. M. Bermejo-Morales, K., Limansky, A.S. Emergence of Pseudomonas aeruginosa Strains Producing Metallo-bata-lactamases of the IMP-15 and VIM-2 types in Mexico. Clinical Microbiology and Infection 16:126-131 (2010).

- Marchiaro, P., Ballerini, V., Spalding, T., Cera, G., Mussi, M.A., Morán Barrio, J., Vila, A.J., Viale, A.M., and Limansky, A.S. A convenient microbiological assay employing cell-free extracts for the rapid characterization of Gram-negative carbapenemase producers. Journal of Antimicrobial Chemotherapy 62: 336-344 (2008).

- Marchiaro, P., Tomatis, P., Mussi, M.A., Pasterán, F., Limansky, A.S., Viale, A.M., Vila, A.J. Biochemical characterization of metallo-β-lactamase VIM-11 from a Pseudomonas aeruginosa clinical strain. Antimicrobial Agents and Chemotherapy 52: 2250-2252 (2008).

-Bantar C, Schell C, Posse G, Limansky A, Ballerini V, Mobilia L. Comparative time-kill study of doxycycline, tigecycline, sulbactam, and imipenem against several clones of Acinetobacter baumannii. Diagn Microbiol Infect Dis 61: 309-314 (2008).

-Marchiaro, P., Mussi, M.A., Ballerini, V., Pasteran, F., Viale, A.M., Vila, A.J., and Limansky, A.S. Sensitive EDTA-Based Microbiological Assays for the Detection of Metallo-β-lactamases in Non-Fermentative Gram-Negative Bacteria. J Clin Microbiol 43: 5648-5352 (2005).

- Mussi, M.A., Limansky, A. and Viale A. Acquisition of Resistance to Carbapenems in Multidrug-Resistant Clinical Strains of Acinetobacter baumannii: Natural insertional inactivation of a gene encoding a member of a novel family of beta-barrel outer membrane proteins. Antimicrob Agents Chemot 49: 1432-1440 (2005).

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Published

2010-05-05

How to Cite

1.
Marchiaro P, Viale AM, Ballerini V, Rossignol G, Vila AJ, Limansky A (2010) First report of a Tn402-like class 1 integron carrying blaVIM-2 in Pseudomonas putida from Argentina. J Infect Dev Ctries 4:412–416. doi: 10.3855/jidc.1012

Issue

Vol. 4 No. 06: June 2010

Section

Letters to the Editor

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