Frequency and susceptibility pattern of metallo-beta-lactamase producers in a hospital in Pakistan

  • Fatima Kaleem Army Medical College, National University of Sciences and Technology, Pakistan
  • Javaid Usman Army Medical College, National University of Sciences and Technology, Pakistan
  • Afreenish Hassan Army Medical College, National University of Sciences and Technology, Pakistan
  • Aslam Khan Army Medical College, National University of Sciences and Technology, Pakistan
Keywords: -resistant Gram-negative rods, metallo-beta-lactamase, antimicrobial resistance, extended-spectrum beta lactamase

Abstract

Introduction: The rapid spread of acquired metallo-beta-lactamases (MBLs) among major Gram-negative pathogens is an emerging threat and a matter of particular concern worldwide.

Methodology: This descriptive study was conducted between January and August 2009 in the department of Microbiology, Army Medical College, National University of Sciences and Technology, Rawalpindi, to determine the frequency and susceptibility patterns of MBL-producers among carbapenem-resistant Gram-negative rods (GNRs) from clinical isolates of a tertiary care hospital. All clinical samples were processed according to standard microbiological methods. Isolated GNRs were subjected to susceptibility testing against various antibiotics by disc diffusion method according to the Clinical and Laboratory Standards Institute (CLSI) guidelines.  Carbapenem-resistant isolates were subjected to the detection of MBL production by the E-test MBL strip method.

Results: Out of 50 carbapenem resistant isolates, 39 (78%) of were confirmed to be MBL producers by the E-strip method.  Acinetobacter baumannii were the most frequent MBL producers, followed by Pseudomonas aeruginosa. A total of 19 (37%) of the MBL producers were susceptible to cefoperazone-sulbactam.

Conclusion: The findings strongly suggest that there is a need to track the detection of MBL producers and that judicious use of carbapenems is necessary to prevent the further spread of these organisms.

Author Biographies

Fatima Kaleem, Army Medical College, National University of Sciences and Technology, Pakistan
Post graduate trainee Microbiology,  National University of Sciences and Technology, Army Medical College,Rawalpindi .Pakistan.
Javaid Usman, Army Medical College, National University of Sciences and Technology, Pakistan
Professor of Pathology, Head department of Microbiology, National University of Sciences and Technology.Army Medical College,  Rawalpindi .Pakistan
Afreenish Hassan, Army Medical College, National University of Sciences and Technology, Pakistan
Post graduate trainee Microbiology,  National University of Sciences and Technology, Army Medical College,Rawalpindi .Pakistan.
Aslam Khan, Army Medical College, National University of Sciences and Technology, Pakistan
Pulmonologist, Military Hospital, Rawalpindi, Pakistan
Published
2010-12-21
How to Cite
1.
Kaleem F, Usman J, Hassan A, Khan A (2010) Frequency and susceptibility pattern of metallo-beta-lactamase producers in a hospital in Pakistan. J Infect Dev Ctries 4:810-813. doi: 10.3855/jidc.1050
Section
Original Articles