Microarray analysis of virulence gene profiles in Salmonella serovars from food/food animal environment

Authors

  • Wen Zou Division of Personalized Nutrition and Medicine, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • Sufian F Al-Khaldi Division of Microbiological Studies, Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, College Park, MD 20740, United States
  • William S Branham Division of Systems Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • Tao Han Division of Systems Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • James C Fuscoe Division of Systems Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • Jing Han Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • Steven L Foley Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • Joshua Xu Z-Tech Corporation, an ICF International company at the National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • Hong Fang Z-Tech Corporation, an ICF International company at the National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • Carl E Cerniglia Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States
  • Rajesh Nayak Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

DOI:

https://doi.org/10.3855/jidc.1396

Keywords:

Salmonella, virulence associated genes, microarray, pathogenicity

Abstract

Introduction: Rapid, accurate and inexpensive analysis of the disease-causing potential of foodborne pathogens is an important consideration in food safety and biodefense, particularly in developing countries.  The objective of this study is to demonstrate the use of a robust and inexpensive microarray platform to assay the virulence gene profiles in Salmonella from food and/or the food animal environment, and then use ArrayTrackTM for data analysis.   

Methodology:  The spotted array consisted of 69 selected Salmonella-specific virulence gene probes (65bp each).  These probes were printed on poly-L-lysine-coated slides.  Genomic DNA was digested with Sau3AI, labeled with Cy3 dye, hybridized to the gene probes, and the images were captured and analyzed by GenePix 4000B and ArrayTrackTM, a free software developed by Food and Drug Administration (FDA) researchers.

Results: Nearly 58% of the virulence-associated genes tested were present in all Salmonella strains tested.  In general, genes belonging to inv, pip, prg, sic, sip, spa or ttr families were detected in more than 90% of the isolates, while the iacP, avrA, invH, rhuM, sirA, sopB, sopE or sugR genes were detected in 40 to 80% of the isolates.  The gene variability was independent of the Salmonella serotype.

Conclusions: This hybridization array presents an accurate and cost-effective method for evaluating the disease-causing potential of Salmonella in outbreak investigations by targeting a selective set of Salmonella-associated virulence genes. 

Author Biographies

Wen Zou, Division of Personalized Nutrition and Medicine, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

Division of Personalized Medicine and Nutrition

Sufian F Al-Khaldi, Division of Microbiological Studies, Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5100 Paint Branch Parkway, College Park, MD 20740, United States

Division of Microbiological Studies

William S Branham, Division of Systems Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

Division of Systems Toxicology

Tao Han, Division of Systems Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

Division of Systems Toxicology

James C Fuscoe, Division of Systems Toxicology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

Division of Systems Toxicology

Jing Han, Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

Division of Microbiology

Steven L Foley, Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

Division of Microbiology

Carl E Cerniglia, Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

Division of Microbiology

Rajesh Nayak, Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, United States

Research Scientist,

Microbiology Division

 

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Published

2010-09-15

How to Cite

1.
Zou W, Al-Khaldi SF, Branham WS, Han T, Fuscoe JC, Han J, Foley SL, Xu J, Fang H, Cerniglia CE, Nayak R (2010) Microarray analysis of virulence gene profiles in Salmonella serovars from food/food animal environment. J Infect Dev Ctries 5:94–105. doi: 10.3855/jidc.1396

Issue

Vol. 5 No. 02: February 2011

Section

Original Articles

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