Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing

Wasun Chantratita; Chonlaphat Sukasem; Sayomporn Sirinavin; Nipaporn Sankuntaw; Chutatip Srichantaratsamee; Ekawat Pasomsub; Kumthorn Malathum

doi:10.3855/jidc.1197

Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing

Authors

Wasun Chantratita Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok
Chonlaphat Sukasem Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok
Sayomporn Sirinavin Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok
Nipaporn Sankuntaw Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok
Chutatip Srichantaratsamee Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok
Ekawat Pasomsub Virology and Molecular Microbiology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Bangkok
Kumthorn Malathum Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok

DOI:

https://doi.org/10.3855/jidc.1197

Keywords:

H274Y, Oseltamivir, Influenza A (H1N1), Pyrosequencing

Abstract

Introduction: We investigated the frequency of H274Y-positive swine-origin 2009 A (H1N1) influenza virus outbreak in Thailand during May-August 2009.

Methodology: This study sought to find Oseltamivir resistance mutation H274Y by using pyrosequencing.

Results: From 8,710 real-time RT-PCR swine-origin 2009 A(H1N1) influenza virus-positive specimens, 100 randomly selected samples identified one such virus with H274Y mutation using pyrosequencing.

Conclusions: The patient probably acquired oseltamivir resistance from natural variation, since he had never received that form of treatment before and recovered from influenza-like symptoms without using anti-influenza drugs.

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Published

2011-03-04

How to Cite

Chantratita W, Sukasem C, Sirinavin S, Sankuntaw N, Srichantaratsamee C, Pasomsub E, Malathum K (2011) Simultaneous detection and subtyping of H274Y-positive influenza A (H1N1) using pyrosequencing. J Infect Dev Ctries 5:348–352. doi: 10.3855/jidc.1197

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Issue

Vol. 5 No. 05: May 2011

Section

Original Articles

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