Identification of feline immunodeficiency virus subtype-B on St. Kitts, West Indies by quantitative PCR

Patrick J Kelly; Ruey Stocking; Dongya Gao; Nikol Phillips; Chuanling Xu; Bernhard Kaltenboeck; Chengming Wang

doi:10.3855/jidc.1844

Identification of feline immunodeficiency virus subtype-B on St. Kitts, West Indies by quantitative PCR

Authors

Patrick J Kelly Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies
Ruey Stocking Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies
Dongya Gao Department of Pathobiology, College of Veterinary Medicine, Auburn University
Nikol Phillips Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies
Chuanling Xu Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies
Bernhard Kaltenboeck Department of Pathobiology, College of Veterinary Medicine, Auburn University
Chengming Wang Ross University School of Veterinary Medicine, Basseterre, St. Kitts, West Indies

DOI:

https://doi.org/10.3855/jidc.1844

Keywords:

feline immunodeficiency virus, fluorescence resonance energy transfer (FRET) PCR, Caribbean

Abstract

Introduction: Although antibodies to the feline immunodeficiency virus (FIV) have been detected by SNAP assay in cats from St. Kitts, there have been no molecular studies to further confirm the infection and determine the FIV subtypes present.

Methodology: Total nucleic acids were extracted from EDTA whole blood specimens from 35 cats, followed by quantitative fluorescence resonance energy transfer (FRET) PCR under a six-channel LightCycler 2.0 Instrument with Software version 4.1.

Results: Four of 11 stray cats (36 %) but none of 24 owned cats were FIV positive by real-time PCR. High-resolution melting curve analysis indicated that all four positive cats were infected with FIV subtype-B.

Conclusions: This is the first molecular characterization of FIV subtypes on St. Kitts and the results confirm the high prevalence of FIV infection in stray cats on the island.

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Published

2011-05-21

How to Cite

Kelly PJ, Stocking R, Gao D, Phillips N, Xu C, Kaltenboeck B, Wang C (2011) Identification of feline immunodeficiency virus subtype-B on St. Kitts, West Indies by quantitative PCR. J Infect Dev Ctries 5:480–483. doi: 10.3855/jidc.1844

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Issue

Vol. 5 No. 06: June 2011

Section

Brief Original Articles

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