The stability of human, bovine and avian tuberculin purified protein derivative (PPD)

  • Mailis Maes Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela
  • José Francisco Giménez Decanato de Ciencias Veterinario Universidad Centrooccidente Lisandro Alvarez (UCLA), Barquisimeto, Venezuela
  • Adriana D’Alessandro Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela
  • Jacobus H. de Waard Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela
Keywords: tuberculin PPD, stability, potency,

Abstract

Introduction: Guidelines recommend storing tuberculin purified protein derivative (PPD) refrigerated. However, especially in developing countries, maintaining the product refrigerated under field conditions can be difficult, limiting its use. Here we determine the effect of prolonged exposure to high temperatures on the potency of human, bovine and avian tuberculin PPD.

Methodology: Human, bovine and avian tuberculin PPD were stored for several weeks exposed to temperatures ranging from 37º to 100ºC. The potency was evaluated in vivo, in sensitized or naturally infected animals.

Results: Most test situations didn't affect the biological activity of the tuberculin PPDs and only very long and extreme incubations (several days at 100°C) compromised the potency.

Conclusions: Tuberculin PPD is very stable and can be stored or transported for long periods without refrigeration. 

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Author Biographies

Mailis Maes, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela
PhD student in the Laboratorio de Tuberculosis,
José Francisco Giménez, Decanato de Ciencias Veterinario Universidad Centrooccidente Lisandro Alvarez (UCLA), Barquisimeto, Venezuela
Veterinarian Laboratorio de Zoonosis “Dr. Angel Meléndez”, Decanato de Ciencias Veterinario
Adriana D’Alessandro, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela

PhD student Laboratorio de Tuberculosis

Jacobus H. de Waard, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela
Chief of the Laboratorio de Tuberculosis Instituto de Biomedicna
Published
2011-11-09
How to Cite
1.
Maes M, Giménez JF, D’Alessandro A, de WaardJH (2011) The stability of human, bovine and avian tuberculin purified protein derivative (PPD). J Infect Dev Ctries 5:781-785. doi: 10.3855/jidc.1689
Section
Original Articles

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