Mycobacterium tuberculosis complex detected by modified fluorescent in situ hybridization in lymph nodes of clinical samples

Authors

  • Juan Rodriguez-Nuñez Universidad Autónoma de Aguascalientes, Aguascalientes, México
  • Francisco J Avelar Universidad Autónoma de Aguascalientes, Aguascalientes, México
  • Francisco Marquez Centenario Hospital Miguel Hidalgo, Aguascalientes, México
  • Bruno Rivas-Santiago Unidad de Investigación Médica-Zacatecas México, IMSS
  • Cesar Quiñones Universidad Autónoma de Aguascalientes, Aguascalientes, México
  • Alma L. Guerrero-Barrera Universidad Autónoma de Aguascalientes, Aguascalientes, México

DOI:

https://doi.org/10.3855/jidc.1752

Keywords:

fluorescent in-situ hybridization, FISH, tuberculosis, Mycobacterium tuberculosis complex, PCR, insertion sequence 6110, peptide nucleic acid, PNA

Abstract

Introduction:  Lymph node tuberculosis (TB) is the leading cause of extrapulmonary tuberculosis and is the most frequently identified type in Aguascalientes, Mexico. Conventional diagnosis has serious limitations for rapid detection of extrapulmonary tuberculosis in clinical samples. Here PCR and modified FISH have been tested as complementary diagnosis methods for extrapulmonary tuberculosis.
Methodology: The specific insertion sequence IS6110 for Mycobacterium tuberculosis complex was used to perform PCR and build DNA and PNA FISH probes (20bp). PCR and modified DNA and PNA FISH assays were performed to evaluate 41 lymph node paraffin-embedded tissue samples, in comparison with the histopathology diagnosis, which was considered the gold standard (22 positive and 19 negative).
Results: In comparison with histopathology diagnosis PCR showed 62.5 % sensitivity and 77.8 % specificity (χ2 = 4.583 p < 0.05). Modified DNA FISH showed 71.4% sensitivity and 84.6% specificity (χ2 = 11.21 p < 0.05). PNA FISH showed 66.7% sensitivity and 60.0% specificity (χ2 = 2.93 p > 0.05). Ziehl Neelsen stain was positive in only four cases of 22 lymph node samples positive to histopathology.  In contrast, PCR and modified DNA FISH were positive in 20 cases of the same group. The negative cases were coincident in all tests.
Conclusions: PCR and DNA FISH showed a significant increase in the number of cases detected and also showed higher sensitivity and specificity compared with data reported by traditional methodology. In developing countries, these techniques could help to complement the early diagnosis and timely treatment of extrapulmonary tuberculosis.

Author Biographies

Juan Rodriguez-Nuñez, Universidad Autónoma de Aguascalientes, Aguascalientes, México

Doctorate Student, Doctorado en Ciencias Biologicas, Universidad Autonoma de Aguascalientes.

Francisco J Avelar, Universidad Autónoma de Aguascalientes, Aguascalientes, México

Professor-Researcher, Departamento de Fisiología y Farmacologia. Universidad Autonoma de Aguascalientes.

Francisco Marquez, Centenario Hospital Miguel Hidalgo, Aguascalientes, México

Physician, Infectology Department. Hospital Miguel Hidalgo, Aguascalientes.

Bruno Rivas-Santiago, Unidad de Investigación Médica-Zacatecas México, IMSS

Researcher, Unidad de Investigación Médica-Zacatecas México, IMSS

Cesar Quiñones, Universidad Autónoma de Aguascalientes, Aguascalientes, México

Professor, Departamento de Morfologia, Universidad Autonoma de Aguascalientes.

Alma L. Guerrero-Barrera, Universidad Autónoma de Aguascalientes, Aguascalientes, México

Professor-Researcher, Departamento Morfologia,Universidad Autonoma de Aguascalientes.

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Published

2011-11-30

How to Cite

1.
Rodriguez-Nuñez J, Avelar FJ, Marquez F, Rivas-Santiago B, Quiñones C, Guerrero-Barrera AL (2011) Mycobacterium tuberculosis complex detected by modified fluorescent in situ hybridization in lymph nodes of clinical samples. J Infect Dev Ctries 6:58–66. doi: 10.3855/jidc.1752

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Section

Original Articles